Western blot abreview for Anti-CPS1 antibody - Liver Mitochondrial Marker

Good
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (liver)
Loading amount
40 µg
Specification
liver
Treatment
17.5 mg/kg retrorsine for 24 h
Gel Running Conditions
Reduced Non-Denaturing (Native) (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/5000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk

Secondary antibody

Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL
Exposure
2 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 150 kDa Non-specific: ~60 kDa

Dr. Junyi Xue

Verified customer

Submitted Jul 03 2012

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