For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
Pls reply us the question from our customer, pls find below Email.
I want to bring to your attention that for the detection of CPS1 as liver mitochondrial activity indicator I bought only anti-CPS1 antibody –liver mitochondrial marker (ab45956) abcam , would you please inform me: to do western blot technique can I use *** HRP kit as an indicator for the anti-CPS1 antibody, as it is available in my lab. This indicator kit consists of : biotinylated link, DAB chromogen, DAB substrate buffer. And streptavidin-HRP.
Thank you in advance for your kind care given to this matter.
Asked on Mar 14 2012
Thank you for contacting us with your customer's question.
The referenced kit is intended to be used with immunohistochemistry staining of tissue, and has not been optimized for Western blotting. It might be possible to get some results in Western blotting with this kit, but the buffers and dilutions are not intended for use in Western blot and the results may not be ideal.
I would recommend using a DAB detection kit that has been developed for use in Western blot, which we do not currently sell but may be available on http://www.biocompare.com. Alternatively if the customer does not want to purchase a full kit,then she could use an HRP-conjugated secondary such as ab6721 (link is below)with just a Western blot DAB reagent.
I hope this information will be useful, but please let me know ifthe customerhas any further questions or if there is anything else that I can do for you, and I'll be happy to help.
Answered on Mar 14 2012