Recombinant
RabMAb

Anti-CPS1 antibody [EPR7493-29] (ab155083)

Overview

  • Product name
    Anti-CPS1 antibody [EPR7493-29]
    See all CPS1 primary antibodies
  • Description
    Rabbit monoclonal [EPR7493-29] to CPS1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human CPS1 (UniProt: P31327).

  • Positive control
    • HeLa, Human fetal liver and Rat liver cell lysates; Human liver tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab155083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 165 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell.
    • Tissue specificity
      Primarily in the liver and small intestine.
    • Involvement in disease
      Defects in CPS1 are the cause of carbamoyl phosphate synthetase 1 deficiency (CPS1D) [MIM:237300]. CPS1D is an autosomal recessive disorder of the urea cycle causing hyperammonemia. Clinical features include protein intolerance, intermittent ataxia, seizures, lethargy, developmental delay and mental retardation.
      Note=Genetic variations in CPS1 influence the availability of precursors for nitric oxide (NO) synthesis and play a role in clinical situations where endogenous NO production is critically important, such as neonatal pulmonary hypertension, increased pulmonary artery pressure following surgical repair of congenital heart defects or hepatovenocclusive disease following bone marrow transplantation. Infants with neonatal pulmonary hypertension homozygous for Thr-1406 have lower L-arginine concentrations than neonates homozygous for Asn-1406.
    • Sequence similarities
      Contains 2 ATP-grasp domains.
      Contains 1 glutamine amidotransferase type-1 domain.
    • Domain
      The type-1 glutamine amidotransferase domain is defective.
    • Cellular localization
      Mitochondrion.
    • Information by UniProt
    • Database links
    • Alternative names
      • Carbamoyl phosphate synthase [ammonia] antibody
      • Carbamoyl phosphate synthase [ammonia] mitochondrial antibody
      • Carbamoyl phosphate synthase antibody
      • Carbamoyl phosphate synthetase 1 antibody
      • Carbamoyl phosphate synthetase 1 mitochondrial antibody
      • Carbamoyl phosphate synthetase I antibody
      • Carbamoyl-phosphate synthase [ammonia] antibody
      • Carbamoyl-phosphate synthetase I antibody
      • Carbamoylphosphate synthase antibody
      • Carbamoylphosphate synthetase 1 antibody
      • Carbamoylphosphate synthetase I antibody
      • CPS 1 antibody
      • Cps1 antibody
      • CPSase 1 antibody
      • CPSase I antibody
      • CPSASE1 antibody
      • CPSM_HUMAN antibody
      • mitochondrial antibody
      • MS738 antibody
      see all

    Images

    • All lanes : Anti-CPS1 antibody [EPR7493-29] (ab155083) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Human fetal liver lysate
      Lane 3 : Rat liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 165 kDa

    • Immunohistochemical analysis of paraffin-embedded Human liver tissue, labeling CPS1 with ab155083 at 1/250 dilution.

    References

    ab155083 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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