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RecombinantRabMAb

Recombinant Anti-CPS1 antibody [EPR7494] (ab128942)

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Western blot - Anti-CPS1 antibody [EPR7494] (ab128942)
  • Flow Cytometry - Anti-CPS1 antibody [EPR7494] (ab128942)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPS1 antibody [EPR7494] (ab128942)
  • Anti-CPS1 antibody [EPR7494] (ab128942)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7494] to CPS1
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

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Protein
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Recombinant Human CPS1 protein (ab152296)

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Overview

  • Product name

    Anti-CPS1 antibody [EPR7494]
    See all CPS1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7494] to CPS1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CPS1 aa 1450-1550. The exact sequence is proprietary.

  • Positive control

    • HeLa cell lysate; Human fetal liver lysate; Human liver tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7494
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism

Associated products

  • Alternative Versions

    • Anti-CPS1 antibody [EPR7494] - BSA and Azide free (ab248236)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human CPS1 protein (ab152296)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab128942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 165 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 165 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.

Target

  • Function

    Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell.
  • Tissue specificity

    Primarily in the liver and small intestine.
  • Involvement in disease

    Defects in CPS1 are the cause of carbamoyl phosphate synthetase 1 deficiency (CPS1D) [MIM:237300]. CPS1D is an autosomal recessive disorder of the urea cycle causing hyperammonemia. Clinical features include protein intolerance, intermittent ataxia, seizures, lethargy, developmental delay and mental retardation.
    Note=Genetic variations in CPS1 influence the availability of precursors for nitric oxide (NO) synthesis and play a role in clinical situations where endogenous NO production is critically important, such as neonatal pulmonary hypertension, increased pulmonary artery pressure following surgical repair of congenital heart defects or hepatovenocclusive disease following bone marrow transplantation. Infants with neonatal pulmonary hypertension homozygous for Thr-1406 have lower L-arginine concentrations than neonates homozygous for Asn-1406.
  • Sequence similarities

    Contains 2 ATP-grasp domains.
    Contains 1 glutamine amidotransferase type-1 domain.
  • Domain

    The type-1 glutamine amidotransferase domain is defective.
  • Cellular localization

    Mitochondrion.
  • Target information above from: UniProt accession P31327 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1373 Human
    • Omim: 608307 Human
    • SwissProt: P31327 Human
    • Unigene: 149252 Human
    • Alternative names

      • Carbamoyl phosphate synthase [ammonia] antibody
      • Carbamoyl phosphate synthase [ammonia] mitochondrial antibody
      • Carbamoyl phosphate synthase antibody
      • Carbamoyl phosphate synthetase 1 antibody
      • Carbamoyl phosphate synthetase 1 mitochondrial antibody
      • Carbamoyl phosphate synthetase I antibody
      • Carbamoyl-phosphate synthase [ammonia] antibody
      • Carbamoyl-phosphate synthetase I antibody
      • Carbamoylphosphate synthase antibody
      • Carbamoylphosphate synthetase 1 antibody
      • Carbamoylphosphate synthetase I antibody
      • CPS 1 antibody
      • Cps1 antibody
      • CPSase 1 antibody
      • CPSase I antibody
      • CPSASE1 antibody
      • CPSM_HUMAN antibody
      • mitochondrial antibody
      • MS738 antibody
      see all

    Images

    • Western blot - Anti-CPS1 antibody [EPR7494] (ab128942)
      Western blot - Anti-CPS1 antibody [EPR7494] (ab128942)
      All lanes : Anti-CPS1 antibody [EPR7494] (ab128942) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Human fetal liver tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 165 kDa
      Observed band size: 150 kDa why is the actual band size different from the predicted?

    • Flow Cytometry - Anti-CPS1 antibody [EPR7494] (ab128942)
      Flow Cytometry - Anti-CPS1 antibody [EPR7494] (ab128942)

      Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling CPS1 with unpurified ab128942 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPS1 antibody [EPR7494] (ab128942)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPS1 antibody [EPR7494] (ab128942)

      ab128942, at 1/250 dilution, staining CPS1 in paraffin-embedded Human liver tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-CPS1 antibody [EPR7494] (ab128942)
      Anti-CPS1 antibody [EPR7494] (ab128942)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab128942? Please let us know so that we can cite the reference in this datasheet.

    ab128942 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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