Recombinant Anti-CPS1 antibody [EPR7494] (ab128942)
![Western blot - Anti-CPS1 antibody [EPR7494] (ab128942)](https://www.abcam.com/ps/products/128/ab128942/Images/ab128942-142818-anti-cps1-antibody-epr7494-western-blot.jpg "Western blot - Anti-CPS1 antibody [EPR7494] (ab128942)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7494] to CPS1
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CPS1 antibody [EPR7494]
See all CPS1 primary antibodies -
Description
Rabbit monoclonal [EPR7494] to CPS1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CPS1 aa 1450-1550. The exact sequence is proprietary.
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Positive control
- HeLa cell lysate; Human fetal liver lysate; Human liver tissue
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7494 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 165 kDa).
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Flow Cyt |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 165 kDa). |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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Flow Cyt
Use at an assay dependent concentration. |
Target
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Function
Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell. -
Tissue specificity
Primarily in the liver and small intestine. -
Involvement in disease
Defects in CPS1 are the cause of carbamoyl phosphate synthetase 1 deficiency (CPS1D) [MIM:237300]. CPS1D is an autosomal recessive disorder of the urea cycle causing hyperammonemia. Clinical features include protein intolerance, intermittent ataxia, seizures, lethargy, developmental delay and mental retardation.
Note=Genetic variations in CPS1 influence the availability of precursors for nitric oxide (NO) synthesis and play a role in clinical situations where endogenous NO production is critically important, such as neonatal pulmonary hypertension, increased pulmonary artery pressure following surgical repair of congenital heart defects or hepatovenocclusive disease following bone marrow transplantation. Infants with neonatal pulmonary hypertension homozygous for Thr-1406 have lower L-arginine concentrations than neonates homozygous for Asn-1406. -
Sequence similarities
Contains 2 ATP-grasp domains.
Contains 1 glutamine amidotransferase type-1 domain. -
Domain
The type-1 glutamine amidotransferase domain is defective. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 1373 Human
- Omim: 608307 Human
- SwissProt: P31327 Human
- Unigene: 149252 Human
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Alternative names
- Carbamoyl phosphate synthase [ammonia] antibody
- Carbamoyl phosphate synthase [ammonia] mitochondrial antibody
- Carbamoyl phosphate synthase antibody
see all
Images
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Western blot - Anti-CPS1 antibody [EPR7494] (ab128942)All lanes : Anti-CPS1 antibody [EPR7494] (ab128942) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Human fetal liver tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 165 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted? -
![Flow Cytometry - Anti-CPS1 antibody [EPR7494] (ab128942)](https://www.abcam.com/ps/products/128/ab128942/Images/ab128942-275856-anti-cps1-antibody-epr7494-flow-cytometry.jpg)
Flow Cytometry - Anti-CPS1 antibody [EPR7494] (ab128942)Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling CPS1 with unpurified ab128942 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPS1 antibody [EPR7494] (ab128942)](https://www.abcam.com/ps/products/128/ab128942/Images/ab128942-142817-anti-cps1-antibody-epr7494-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPS1 antibody [EPR7494] (ab128942)ab128942, at 1/250 dilution, staining CPS1 in paraffin-embedded Human liver tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-CPS1 antibody [EPR7494] (ab128942)](https://www.abcam.com/ps/products/128/ab128942/Images/ab128942-4-benefits-of-recombinant-antibodies.png)
Anti-CPS1 antibody [EPR7494] (ab128942)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab128942 has not yet been referenced specifically in any publications.