Key features and details
- Rabbit polyclonal to CPSF1
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CPSF1 antibody
See all CPSF1 primary antibodies
DescriptionRabbit polyclonal to CPSF1
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
Synthetic peptide corresponding to a region between residue 1392 and 1442 of Human CPSF1 (NP_037423.1).
- HeLa, 293T and NIH3T3 whole cell lysates.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab81552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 161 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
RelevanceCPSF1 (Cleavage and polyadenylation specificity factor subunit 1) is a component of the cleavage and polyadenylation specificity factor (CPSF) complex. It plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction.
Cellular localizationNucleus, nucleoplasm.
- Cleavage and polyadenylation specificity factor 160 kDa subunit antibody
- Cleavage and polyadenylation specificity factor subunit 1 antibody
- CPSF 160 kDa subunit antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling CPSF1 with ab81552 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-CPSF1 antibody (ab81552) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 161 kDa
Observed band size: 171 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
HeLa cell lysate (1mg) was immunoprecipitated using ab81552 at 3µg/mg lysate (lane 1) or IgG control (lane 2). 20% of the immunoprecipitate was analysed by Western blot using ab81552 at 1µg/ml. Bands were detected by chemiluminescence with an exposure time of 10 seconds.
IHC image of ab81552 staining in Human Breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab81552, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab81552 has been referenced in 3 publications.
- Takahashi H et al. The role of Mediator and Little Elongation Complex in transcription termination. Nat Commun 11:1063 (2020). PubMed: 32102997
- Sakai A et al. Aberrant expression of CPSF1 promotes head and neck squamous cell carcinoma via regulating alternative splicing. PLoS One 15:e0233380 (2020). PubMed: 32437477
- Yu S et al. SWI/SNF interacts with cleavage and polyadenylation factors and facilitates pre-mRNA 3' end processing. Nucleic Acids Res 46:8557-8573 (2018). PubMed: 29860334