Overview

  • Product name

  • Description

    Rabbit polyclonal to CPSF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within the amino acids 1392-1442 of Human CPSF1, NP_037423.1

  • Positive control

    • Human anaplastic thyroid carcinoma, bladder cell carcinoma, breast carcinoma, colon carcinoma, linitis plastica stomach cancer, ovarian carcinoma, prostate carcinoma, skin basal cell carcinoma, small cell lung cancer, stomach adenocarcinoma, testicular seminoma. Mouse colon carcinoma CT26, renal cell carcinoma, teratoma.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibody was affinity purified using an epitope specific to CPSF160 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-P
  • Application notes
    ICC/IF: 1/100.
    IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate will enhance staining.

    Likely to work with frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      CPSF1 (Cleavage and polyadenylation specificity factor subunit 1) is a component of the cleavage and polyadenylation specificity factor (CPSF) complex. It plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction.
    • Cellular localization

      Nucleus, nucleoplasm.
    • Database links

    • Alternative names

      • Cleavage and polyadenylation specificity factor 160 kDa subunit antibody
      • Cleavage and polyadenylation specificity factor subunit 1 antibody
      • CPSF 160 kDa subunit antibody
      • CPSF160 antibody
      • HSU37012 antibody
      • P/cl.18 antibody
      see all

    Images

    • Detection of Human CPSF1 by IHC-IF.
      Sample: FFPE section of Human breast carcinoma.
      Antibody: ab84743 used at a dilution of 1/100.
      Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1/100.
    • Detection of Human CPSF1 by Immunohistochemistry.
      Sample: FFPE section of Human breast carcinoma.
      Antibody: ab84743 used at a dilution of 1/100.
      Detection: DAB staining.
    • Detection of Human CPSF1 by Immunohistochemistry.
      Sample: FFPE section of Human prostate carcinoma.
      Antibody: ab84743 used at a dilution of 1/500.
      Detection: DAB staining.
    • Detection of Mouse CPSF1 by Immunohistochemistry.
      Sample: FFPE section of Mouse teratoma.
      Antibody: ab84743 used at a dilution of 1/100.
      Detection: DAB staining.

    References

    ab84743 has not yet been referenced specifically in any publications.

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