1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
CPSF2 belongs to the RNA-metabolizing metallo-beta-lactamase-like family, CPSF2/YSH1 subfamily. It is a component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3 prime-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. A poly(A) tail is added to eukaryotic mRNA precursors after tightly coupled cleavage and polyadenylation reactions at their 3-prime ends.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreatic islet cell tumor (left) and mouse teratoma (right) tissues labelling CPSF2 with ab81553 at 1/200 (1µg/ml). Detection: DAB.
Western blot - Anti-CPSF2 antibody (ab81553)
All lanes : Anti-CPSF2 antibody (ab81553) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
HeLa cell lysate (1mg) was immunoprecipitated using ab81553 at 3µg/mg (lane 1) or IgG control (lane 2). 20% of the immunoprecipitate was analysed by Western blotting using ab81553 at 1µg/ml. Bands were observed by chemiluminescence with a 3 second exposure time.