Overview

  • Product name

    Anti-CPSF2/CPSF100 antibody
    See all CPSF2/CPSF100 primary antibodies
  • Description

    Rabbit polyclonal to CPSF2/CPSF100
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Zebrafish, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human CPSF2/CPSF100 (internal sequence). The exact sequence is proprietary.
    Database link: Q9P2I0

  • Positive control

    • WB: U-87 MG whole cell lysate. ICC/IF: NTERA-2 cl.D1 [NT2/D1] cells.
  • General notes

     This product was previously labelled as CPSF2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab229114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 88 kDa.

Target

Images

  • Anti-CPSF2/CPSF100 antibody (ab229114) at 1/1000 dilution + U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg

    Predicted band size: 88 kDa



    7.5% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) cells stained for CPSF2/CPSF100 (green) using ab229114 at 1/500 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab229114 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Zebrafish Cell lysate - nuclear (3dpf larvae)
Gel Running Conditions
Reduced Denaturing (NuPAGE 4-12% bis Tris gel)
Loading amount
40 µg
Specification
3dpf larvae
Blocking step
Odyssey as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Aug 01 2019

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