Key features and details
- Rabbit polyclonal to CPSF2/CPSF100
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CPSF2/CPSF100 antibody
See all CPSF2/CPSF100 primary antibodies
DescriptionRabbit polyclonal to CPSF2/CPSF100
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Xenopus tropicalis
Synthetic peptide corresponding to Human CPSF2/CPSF100.
Database link: NP_059133.1
- HeLa, 293T and NIH3T3 whole cell lysates.
This product was previously labelled as CPSF2
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab81553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 88 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionComponent of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Involved in the histone 3' end pre-mRNA processing.
Sequence similaritiesBelongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF2/YSH1 subfamily.
- Information by UniProt
- Cleavage and polyadenylation specific factor 2 antibody
- cleavage and polyadenylation specific factor 2, 100kDa antibody
- Cleavage and polyadenylation specificity factor 100 kDa subunit antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreatic islet cell tumor (left) and mouse teratoma (right) tissues labelling CPSF2/CPSF100 with ab81553 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-CPSF2/CPSF100 antibody (ab81553) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 88 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
HeLa cell lysate (1mg) was immunoprecipitated using ab81553 at 3µg/mg (lane 1) or IgG control (lane 2). 20% of the immunoprecipitate was analysed by Western blotting using ab81553 at 1µg/ml. Bands were observed by chemiluminescence with a 3 second exposure time.
ab81553 has not yet been referenced specifically in any publications.