Overview

  • Product name

    Anti-CPSF2/CPSF100 antibody
    See all CPSF2/CPSF100 primary antibodies
  • Description

    Rabbit polyclonal to CPSF2/CPSF100
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Platypus
  • Immunogen

    Synthetic peptide corresponding to Human CPSF2/CPSF100.
    Database link: NP_059133.1

  • Positive control

    • HeLa, 293T or NIH3T3 whole cell lysate
  • General notes

     This product was previously labelled as CPSF2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab81554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 88 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Involved in the histone 3' end pre-mRNA processing.
  • Sequence similarities

    Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF2/YSH1 subfamily.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cleavage and polyadenylation specific factor 2 antibody
    • cleavage and polyadenylation specific factor 2, 100kDa antibody
    • Cleavage and polyadenylation specificity factor 100 kDa subunit antibody
    • Cleavage and polyadenylation specificity factor subunit 2 antibody
    • CPSF 100 kDa subunit antibody
    • CPSF 2 antibody
    • CPSF100 antibody
    • Cpsf2 antibody
    • CPSF2_HUMAN antibody
    • KIAA1367 antibody
    • zgc:92484 antibody
    see all

Images

  • All lanes : Anti-CPSF2/CPSF100 antibody (ab81554) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 88 kDa
    Observed band size: 88 kDa
    Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 seconds
  • HeLa whole cell lysate (1mg) was immunoprecipitated with ab81554 at 3µg/mg lysate (lane 1) or control IgG (lane 2). 20% of the immunoprecipitate was analysed by Western blot using ab81554 at 1µg/ml.

References

ab81554 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab81554.
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