Overview

  • Product name

    Anti-CPSF2/CPSF100 antibody [EPR7599]
    See all CPSF2/CPSF100 primary antibodies
  • Description

    Rabbit monoclonal [EPR7599] to CPSF2/CPSF100
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CPSF2/CPSF100 aa 700-800. The exact sequence is proprietary.

  • Positive control

    • HeLa, Ramos, Jurkat, and 293T cell lysates.
  • General notes

     

     

     This product was previously labelled as CPSF2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 103 kDa (predicted molecular weight: 88 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function

      Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Involved in the histone 3' end pre-mRNA processing.
    • Sequence similarities

      Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF2/YSH1 subfamily.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cleavage and polyadenylation specific factor 2 antibody
      • cleavage and polyadenylation specific factor 2, 100kDa antibody
      • Cleavage and polyadenylation specificity factor 100 kDa subunit antibody
      • Cleavage and polyadenylation specificity factor subunit 2 antibody
      • CPSF 100 kDa subunit antibody
      • CPSF 2 antibody
      • CPSF100 antibody
      • Cpsf2 antibody
      • CPSF2_HUMAN antibody
      • KIAA1367 antibody
      • zgc:92484 antibody
      see all

    Images

    • All lanes : Anti-CPSF2/CPSF100 antibody [EPR7599] (ab126760) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Ramos cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution

      Predicted band size: 88 kDa

    References

    This product has been referenced in:

    • Yu S  et al. SWI/SNF interacts with cleavage and polyadenylation factors and facilitates pre-mRNA 3' end processing. Nucleic Acids Res 46:8557-8573 (2018). Read more (PubMed: 29860334) »
    • Guillén-Boixet J  et al. CPEB4 is regulated during cell cycle by ERK2/Cdk1-mediated phosphorylation and its assembly into liquid-like droplets. Elife 5:N/A (2016). Read more (PubMed: 27802129) »
    See all 2 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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