Key features and details
- Rabbit polyclonal to CPSF30/NAR
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CPSF30/NAR antibody
See all CPSF30/NAR primary antibodies
DescriptionRabbit polyclonal to CPSF30/NAR
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Xenopus tropicalis
Recombinant fragment corresponding to Human CPSF30/NAR aa 1-244.
MQEIIASVDHIKFDLEIAVEQQLGAQPLPFPGMDKSGAAVCEFFLKAACG KGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFYS KFGECSNKECPFLHIDPESKIKDCPWYDRGFCKHGPLCRHRHTRRVICVN YLVGFCPEGPSCKFMHPRFELPMGTTEQPPLPQQTQPPAKQSNNPPLQRS SSLIQLTSQNSSPNQQRTPQVIGVMQSQNSSAGNRGPRPLEQVT
Database link: O95639
- WB: HEK-293T whole cell lysate. IHC-P: Human kidney and endometrial cancer tissue.
This product was previously labelled as CPSF30
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230047 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 31,28 kDa.|
|IHC-P||1/20 - 1/200.|
FunctionComponent of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. CPSF4 binds RNA polymers with a preference for poly(U).
Sequence similaritiesBelongs to the CPSF4/YTH1 family.
Contains 5 C3H1-type zinc fingers.
Contains 1 CCHC-type zinc finger.
- Information by UniProt
- cleavage and polyadenylation specific factor 4, 30kDa antibody
- Cleavage and polyadenylation specificity factor 30 kDa subunit antibody
- Cleavage and polyadenylation specificity factor subunit 4 antibody
Anti-CPSF30/NAR antibody (ab230047) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 31,28 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Paraffin-embedded human endometrial cancer tissue stained for CPSF30/NAR using ab230047 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for CPSF30/NAR using ab230047 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230047 has not yet been referenced specifically in any publications.