Product nameAnti-CPSF6 antibody
See all CPSF6 primary antibodies
DescriptionRabbit polyclonal to CPSF6
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig
Recombinant fragment within Human CPSF6 aa 1-194. The exact sequence is proprietary.
Database link: Q16630
- 293T , A431 , HeLa and HepG2 whole cell lysates; mouse colon and rat hind brain tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab184005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 59 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionComponent of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3'-processing. Involved in association with NUDT21/CPSF5 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. CPSF6 binds to cleavage and polyadenylation RNA substrates and promotes RNA looping.
Sequence similaritiesBelongs to the RRM CPSF6/7 family.
Contains 1 RRM (RNA recognition motif) domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. In punctate subnuclear structures localized adjacent to nuclear speckles, called paraspeckles.
- Information by UniProt
- CFIM antibody
- CFIm68 antibody
- cleavage and polyadenylation specific factor 6, 68kDa antibody
All lanes : Anti-CPSF6 antibody (ab184005) at 1/5000 dilution
Lane 1 : 293T cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 59 kDa
7.5% SDS PAGE
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling CPSF6 with ab184005 at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded rat hind brain tissue labeling CPSF6 with ab184005 at 1/500 dilution.
ab184005 has not yet been referenced specifically in any publications.