Anti-CPSF6 antibody (ab99347)
Key features and details
- Rabbit polyclonal to CPSF6
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CPSF6 antibody
See all CPSF6 primary antibodies -
Description
Rabbit polyclonal to CPSF6 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Guinea pig, Cow, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan, Xenopus tropicalis, Medaka fish -
Immunogen
Synthetic peptide, corresponding to a region between residue 1 and 50 of Human CPSF6 (NP_008938.1).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab99347 was affinity purified using an epitope specific to CPSF6 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 59 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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IHC-P
Use at an assay dependent concentration. |
WB
1/2000 - 1/10000. Predicted molecular weight: 59 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3'-processing. Involved in association with NUDT21/CPSF5 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. CPSF6 binds to cleavage and polyadenylation RNA substrates and promotes RNA looping. -
Sequence similarities
Belongs to the RRM CPSF6/7 family.
Contains 1 RRM (RNA recognition motif) domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. In punctate subnuclear structures localized adjacent to nuclear speckles, called paraspeckles. - Information by UniProt
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Database links
- Entrez Gene: 417844 Chicken
- Entrez Gene: 768231 Cow
- Entrez Gene: 11052 Human
- Entrez Gene: 432508 Mouse
- Entrez Gene: 100174771 Orangutan
- Entrez Gene: 299811 Rat
- Entrez Gene: 446580 Xenopus laevis
- Entrez Gene: 327069 Zebrafish
see all -
Alternative names
- CFIM antibody
- CFIm68 antibody
- cleavage and polyadenylation specific factor 6, 68kDa antibody
see all
Images
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All lanes : Anti-CPSF6 antibody (ab99347) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 59 kDa
Exposure time: 10 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling CPSF6 with ab99347 at 1/200 (1µg/ml). Detection: DAB.
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Detection of CPSF6 by Western Blot of Immunprecipitate. ab99347 at 1µg/ml staining CPSF6 in HeLa whole cell lysate immunoprecipitated using ab99347 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 1 seconds.
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IHC image of CPSF6 staining in human breast fibroadenoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab99347, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab99347 has been referenced in 8 publications.
- Schwich OD et al. SRSF3 and SRSF7 modulate 3'UTR length through suppression or activation of proximal polyadenylation sites and regulation of CFIm levels. Genome Biol 22:82 (2021). PubMed: 33706811
- Chen S et al. The mRNA Export Receptor NXF1 Coordinates Transcriptional Dynamics, Alternative Polyadenylation, and mRNA Export. Mol Cell 74:118-131.e7 (2019). PubMed: 30819645
- Bochnakian A et al. Interferon-Inducible MicroRNA miR-128 Modulates HIV-1 Replication by Targeting TNPO3 mRNA. J Virol 93:N/A (2019). PubMed: 31341054
- Bulli L et al. Complex Interplay between HIV-1 Capsid and MX2-Independent Alpha Interferon-Induced Antiviral Factors. J Virol 90:7469-80 (2016). PubMed: 27279606
- Dharan A et al. KIF5B and Nup358 Cooperatively Mediate the Nuclear Import of HIV-1 during Infection. PLoS Pathog 12:e1005700 (2016). PubMed: 27327622
- Rasheedi S et al. The Cleavage and Polyadenylation Specificity Factor 6 (CPSF6) Subunit of the Capsid-recruited Pre-messenger RNA Cleavage Factor I (CFIm) Complex Mediates HIV-1 Integration into Genes. J Biol Chem 291:11809-19 (2016). PubMed: 26994143
- Rasaiyaah J et al. HIV-1 evades innate immune recognition through specific cofactor recruitment. Nature 503:402-5 (2013). WB ; Human . PubMed: 24196705
- Hori T et al. A carboxy-terminally truncated human CPSF6 lacking residues encoded by exon 6 inhibits HIV-1 cDNA synthesis and promotes capsid disassembly. J Virol 87:7726-36 (2013). WB ; Human . PubMed: 23658440