Recombinant
RabMAb

Recombinant Anti-CPSF6 antibody [EPR12898] - BSA and Azide free (ab238933)

Overview

  • Product name

    Anti-CPSF6 antibody [EPR12898] - BSA and Azide free
    See all CPSF6 primary antibodies
  • Description

    Rabbit monoclonal [EPR12898] to CPSF6 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-P, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Xenopus laevis
  • Immunogen

    Synthetic peptide within Human CPSF6 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q16630

  • Positive control

    • IHC-P: Human breast tissue.
  • General notes

    Ab238933 is the carrier-free version of ab175237. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab238933 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12898
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab238933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 59 kDa.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3'-processing. Involved in association with NUDT21/CPSF5 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. CPSF6 binds to cleavage and polyadenylation RNA substrates and promotes RNA looping.
  • Sequence similarities

    Belongs to the RRM CPSF6/7 family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. In punctate subnuclear structures localized adjacent to nuclear speckles, called paraspeckles.
  • Information by UniProt
  • Database links

  • Alternative names

    • CFIM antibody
    • CFIm68 antibody
    • cleavage and polyadenylation specific factor 6, 68kDa antibody
    • Cleavage and polyadenylation specificity factor 68 kDa subunit antibody
    • Cleavage and polyadenylation specificity factor subunit 6 antibody
    • cleavage factor Im complex 68 kDa subunit antibody
    • CPSF 68 kDa subunit antibody
    • Cpsf6 antibody
    • CPSF6_HUMAN antibody
    • HPBRII 4 antibody
    • HPBRII 7 antibody
    • Pre mRNA cleavage factor Im 68 kDa subunit antibody
    • pre-mRNA cleavage factor I, 68kD subunit antibody
    • pre-mRNA cleavage factor Im (68kD) antibody
    • Pre-mRNA cleavage factor Im 68 kDa subunit antibody
    • Protein HPBRII 4/7 antibody
    • Protein HPBRII-4/7 antibody
    see all

Images

  • Immunoprecipitation of CPSF6 from HeLa cell lysate using ab175237 at 1/10 labeled with ab175237 at 1/10.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175237).

  • Immunofluorescent analysis of HeLa cells labeling CPSF6 with ab175237 at 1/100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175237).

  • Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling CPSF6 with ab175237 at 1/100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175237).

  • Flow Cytometrical analysis of permeabilized Jurakt cells labeling CPSF6 with ab175237 antibody at 1/10 (red) or a rabbit IgG negative (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175237).

  • Immunohistochemical analysis of paraffin embedded Human breast tissue labeling CPSF6 with ab175237 at 1/100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175237).

References

ab238933 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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