Recombinant
RabMAb

Anti-CPSF6 antibody [EPR12899] (ab181221)

Overview

  • Product name
    Anti-CPSF6 antibody [EPR12899]
    See all CPSF6 primary antibodies
  • Description
    Rabbit monoclonal [EPR12899] to CPSF6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CPSF6 aa 150-250. The exact sequence is proprietary.
    Database link: Q16630

  • Positive control
    • HeLa, 293T, Jurkat and K562 cell lysate. Human testis tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR12899
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 59 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3'-processing. Involved in association with NUDT21/CPSF5 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. CPSF6 binds to cleavage and polyadenylation RNA substrates and promotes RNA looping.
  • Sequence similarities
    Belongs to the RRM CPSF6/7 family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. In punctate subnuclear structures localized adjacent to nuclear speckles, called paraspeckles.
  • Information by UniProt
  • Database links
  • Alternative names
    • CFIM antibody
    • CFIm68 antibody
    • cleavage and polyadenylation specific factor 6, 68kDa antibody
    • Cleavage and polyadenylation specificity factor 68 kDa subunit antibody
    • Cleavage and polyadenylation specificity factor subunit 6 antibody
    • cleavage factor Im complex 68 kDa subunit antibody
    • CPSF 68 kDa subunit antibody
    • Cpsf6 antibody
    • CPSF6_HUMAN antibody
    • HPBRII 4 antibody
    • HPBRII 7 antibody
    • Pre mRNA cleavage factor Im 68 kDa subunit antibody
    • pre-mRNA cleavage factor I, 68kD subunit antibody
    • pre-mRNA cleavage factor Im (68kD) antibody
    • Pre-mRNA cleavage factor Im 68 kDa subunit antibody
    • Protein HPBRII 4/7 antibody
    • Protein HPBRII-4/7 antibody
    see all

Images

  • All lanes : Anti-CPSF6 antibody [EPR12899] (ab181221) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

    Predicted band size: 59 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Immunohistochemical analysis of paraffin embedded Human breast cancer tissue sections labeling CPSF6 using ab181221 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

  • Immunohistochemical analysis of paraffin embedded Human testis tissue sections labeling CPSF6 using ab181221 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

References

ab181221 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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