Product nameAnti-CPSF7 antibody
See all CPSF7 primary antibodies
DescriptionRabbit polyclonal to CPSF7
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- WB: NIH/3T3, NBT-II cell lysate. RT4 IHC-P: Human esophagus tissue. ICC/IF: A431.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab272634 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
FunctionComponent of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. Binds to cleavage and polyadenylation RNA substrates.
Sequence similaritiesBelongs to the RRM CPSF6/7 family.
Contains 1 RRM (RNA recognition motif) domain.
- Information by UniProt
- CFIm59 antibody
- Cleavage and polyadenylation specificity factor 59 kDa subunit antibody
- Cleavage and polyadenylation specificity factor 7 antibody
All lanes : Anti-CPSF7 antibody (ab272634) at 0.4 µg/ml
Lane 1 : NIH/3T3 cell lysate
Lane 2 : NBT-II cell lysate
Lane 2 : Anti-CPSF7 antibody (ab272634) at 0.4 µg/ml
Lane 1 : Molecular weight ladder
Lane 2 : RT4
Paraffin-embedded formalin-fixed Human esophagus tissue stained for CPSF7 using ab272634 at 1/50 in immunohistochemical analysis.
PFA fixed, Triton X-100 permeablized human cell line A431 labeling CPSF7 (green) using ab272634 at 2 ug/ml in ICC/IF analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272634 has not yet been referenced specifically in any publications.