Product nameAnti-CPSF7 antibody
See all CPSF7 primary antibodies
DescriptionRabbit polyclonal to CPSF7
Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog
- WB: HepG2 cell lysate. IHC-P: Human lung tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab50965 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/62500.
FunctionComponent of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. Binds to cleavage and polyadenylation RNA substrates.
Sequence similaritiesBelongs to the RRM CPSF6/7 family.
Contains 1 RRM (RNA recognition motif) domain.
- Information by UniProt
- CFIm59 antibody
- Cleavage and polyadenylation specificity factor 59 kDa subunit antibody
- Cleavage and polyadenylation specificity factor 7 antibody
Lane 2 : Anti-CPSF7 antibody (ab50965) at 1.25 µg/ml
Lane 1 : Marker
Lane 2 : HepG2 cell lysate at 10 µg
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50,000
Predicted band size: 52 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
ab50965, at a concentration of 4-8 µg/ml, staining CPSF7 in paraffin embedded human lung tissue sections by Immunohistochemistry. Cells with positive label are Aveolar cells (indicated by arrows). Magnification: 400x.