Anti-CPSF73 antibody (ab154596)

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Overview

  • Product name

    Anti-CPSF73 antibody
    See all CPSF73 primary antibodies

  • Description

    Rabbit polyclonal to CPSF73

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis

  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 74-306 of Human CPSF73 (UniProt ID: Q9UKF6).

  • Positive control

    • 293T, A431 whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab154596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 77 kDa.

Target

  • Function

    Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner.

  • Sequence similarities

    Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF3 subfamily.

  • Post-translational
    modifications

    Sumoylated on Lys-462, Lys-465 and Lys-545, preferentially by SUMO3.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Cleavage and polyadenylation specific factor 3 73kDa antibody
    • Cleavage and polyadenylation specificity factor 73 kDa subunit antibody
    • Cleavage and polyadenylation specificity factor subunit 3 antibody
    • CPSF 73 antibody
    • CPSF 73 kDa subunit antibody
    • CPSF antibody
    • CPSF-73 antibody
    • cpsf3 antibody
    • CPSF3_HUMAN antibody
    • CPSF73 antibody
    • mRNA 3''-end-processing endonuclease CPSF-73 antibody
    • YSH1 antibody
    see all

Images

  • Western blot - Anti-CPSF73 antibody (ab154596)
    Western blot - Anti-CPSF73 antibody (ab154596)
    Anti-CPSF73 antibody (ab154596) at 1/2000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 77 kDa



    7.5% SDS PAGE

References

ab154596 has not yet been referenced specifically in any publications.

Publishing research using ab154596? Please let us know so that we can cite the reference in this datasheet.

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