Anti-CPSF73 antibody (ab154596)

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Western blot - Anti-CPSF73 antibody (ab154596)

    Key features and details

    • Rabbit polyclonal to CPSF73
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-CPSF73 antibody
      See all CPSF73 primary antibodies

    • Description

      Rabbit polyclonal to CPSF73

    • Host species

      Rabbit

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis

    • Immunogen

      Recombinant fragment, corresponding to a region within amino acids 74-306 of Human CPSF73 (UniProt ID: Q9UKF6).

    • Positive control

      • 293T, A431 whole cell lysate

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab154596 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/3000. Predicted molecular weight: 77 kDa.

    Target

    • Function

      Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner.

    • Sequence similarities

      Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF3 subfamily.

    • Post-translational
      modifications

      Sumoylated on Lys-462, Lys-465 and Lys-545, preferentially by SUMO3.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Cleavage and polyadenylation specific factor 3 73kDa antibody
      • Cleavage and polyadenylation specificity factor 73 kDa subunit antibody
      • Cleavage and polyadenylation specificity factor subunit 3 antibody
      • CPSF 73 antibody
      • CPSF 73 kDa subunit antibody
      • CPSF antibody
      • CPSF-73 antibody
      • cpsf3 antibody
      • CPSF3_HUMAN antibody
      • CPSF73 antibody
      • mRNA 3''-end-processing endonuclease CPSF-73 antibody
      • YSH1 antibody
      see all

    Images

    • Western blot - Anti-CPSF73 antibody (ab154596)
      Western blot - Anti-CPSF73 antibody (ab154596)
      Anti-CPSF73 antibody (ab154596) at 1/2000 dilution + A431 whole cell lysate at 30 µg

      Predicted band size: 77 kDa



      7.5% SDS PAGE

    References (0)

    Publishing research using ab154596? Please let us know so that we can cite the reference in this datasheet.

    ab154596 has not yet been referenced specifically in any publications.

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