Product nameAnti-CPSF73 antibody [EPR7468]
See all CPSF73 primary antibodies
DescriptionRabbit monoclonal [EPR7468] to CPSF73
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human CPSF73 aa 650-750. The exact sequence is proprietary.
- WB: HeLa, 293T, Jurkat, Caco-2, RAW 264.7, PC-12, and NIH/3T3 cell lysates. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.70 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab131245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 77 kDa.|
|ICC/IF||1/50 - 1/100.|
FunctionComponent of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner.
Sequence similaritiesBelongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF3 subfamily.
modificationsSumoylated on Lys-462, Lys-465 and Lys-545, preferentially by SUMO3.
- Information by UniProt
- Cleavage and polyadenylation specific factor 3 73kDa antibody
- Cleavage and polyadenylation specificity factor 73 kDa subunit antibody
- Cleavage and polyadenylation specificity factor subunit 3 antibody
All lanes : Anti-CPSF73 antibody [EPR7468] (ab131245) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Caco-2 cell lysate
Lane 5 : RAW 264.7 cell lysate
Lane 6 : PC-12 cell lysate
Lane 7 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 77 kDa
Immunofluorescence analysis of HeLa cells labelling CPSF73 with ab131245 at 1/50 dilution.
Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling CPSF73 (red) with ab131245 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.
Anti-CPSF73 antibody [EPR7468] (ab131245) at 1/1000 dilution + U2OS whole cell lysate at 100 µg
Predicted band size: 77 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
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This product has been referenced in:
- Davidson L et al. Rapid Depletion of DIS3, EXOSC10, or XRN2 Reveals the Immediate Impact of Exoribonucleolysis on Nuclear RNA Metabolism and Transcriptional Control. Cell Rep 26:2779-2791.e5 (2019). Read more (PubMed: 30840897) »