Overview

  • Product name

    Anti-CPSF73 antibody [EPR7468]
    See all CPSF73 primary antibodies
  • Description

    Rabbit monoclonal [EPR7468] to CPSF73
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CPSF73 aa 650-750. The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, 293T, Jurkat, Caco-2, RAW 264.7, PC-12, and NIH/3T3 cell lysates. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab131245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 77 kDa.
Flow Cyt 1/250.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner.
    • Sequence similarities

      Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF3 subfamily.
    • Post-translational
      modifications

      Sumoylated on Lys-462, Lys-465 and Lys-545, preferentially by SUMO3.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cleavage and polyadenylation specific factor 3 73kDa antibody
      • Cleavage and polyadenylation specificity factor 73 kDa subunit antibody
      • Cleavage and polyadenylation specificity factor subunit 3 antibody
      • CPSF 73 antibody
      • CPSF 73 kDa subunit antibody
      • CPSF antibody
      • CPSF-73 antibody
      • cpsf3 antibody
      • CPSF3_HUMAN antibody
      • CPSF73 antibody
      • mRNA 3''-end-processing endonuclease CPSF-73 antibody
      • YSH1 antibody
      see all

    Images

    • All lanes : Anti-CPSF73 antibody [EPR7468] (ab131245) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : Caco-2 cell lysate
      Lane 5 : RAW 264.7 cell lysate
      Lane 6 : PC-12 cell lysate
      Lane 7 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 77 kDa

    • Immunofluorescence analysis of HeLa cells labelling CPSF73 with ab131245 at 1/50 dilution.
    • Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling CPSF73 (red) with ab131245 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.

    • Anti-CPSF73 antibody [EPR7468] (ab131245) at 1/1000 dilution + U2OS whole cell lysate at 100 µg

      Predicted band size: 77 kDa

      See Abreview

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Davidson L  et al. Rapid Depletion of DIS3, EXOSC10, or XRN2 Reveals the Immediate Impact of Exoribonucleolysis on Nuclear RNA Metabolism and Transcriptional Control. Cell Rep 26:2779-2791.e5 (2019). Read more (PubMed: 30840897) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2OS)
    Permeabilization
    No
    Specification
    U2OS
    Fixative
    Paraformaldehyde

    Dr. Melanie Blasius

    Verified customer

    Submitted Jun 12 2015

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2OS cells)
    Loading amount
    100 µg
    Specification
    U2OS cells
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Jul 20 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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