Recombinant Anti-CPSF73 antibody [EPR7468] (ab131245)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7468] to CPSF73
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CPSF73 antibody [EPR7468]
See all CPSF73 primary antibodies -
Description
Rabbit monoclonal [EPR7468] to CPSF73 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human CPSF73 aa 650-750. The exact sequence is proprietary.
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Positive control
- WB: HeLa, 293T, Jurkat, Caco-2, RAW 264.7, PC-12, and NIH/3T3 cell lysates. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.70 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7468 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/250.
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 77 kDa.
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ICC/IF | (1) |
1/50 - 1/100.
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Notes |
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Flow Cyt (Intra)
1/250. |
WB
1/1000 - 1/10000. Predicted molecular weight: 77 kDa. |
ICC/IF
1/50 - 1/100. |
Target
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Function
Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. -
Sequence similarities
Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF3 subfamily. -
Post-translational
modificationsSumoylated on Lys-462, Lys-465 and Lys-545, preferentially by SUMO3. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 51692 Human
- Entrez Gene: 54451 Mouse
- Entrez Gene: 298916 Rat
- Omim: 606029 Human
- SwissProt: Q9UKF6 Human
- SwissProt: Q9QXK7 Mouse
- Unigene: 515972 Human
- Unigene: 356778 Mouse
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Alternative names
- Cleavage and polyadenylation specific factor 3 73kDa antibody
- Cleavage and polyadenylation specificity factor 73 kDa subunit antibody
- Cleavage and polyadenylation specificity factor subunit 3 antibody
see all
Images
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All lanes : Anti-CPSF73 antibody [EPR7468] (ab131245) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Caco-2 cell lysate
Lane 5 : RAW 264.7 cell lysate
Lane 6 : PC-12 cell lysate
Lane 7 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 77 kDa -
Immunofluorescence analysis of HeLa cells labelling CPSF73 with ab131245 at 1/50 dilution.
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Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling CPSF73 (red) with ab131245 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.
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Anti-CPSF73 antibody [EPR7468] (ab131245) at 1/1000 dilution + U2OS whole cell lysate at 100 µg
Predicted band size: 77 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab131245 has been referenced in 2 publications.
- Yang L et al. SP1 induced long non-coding RNA LINC00958 overexpression facilitate cell proliferation, migration and invasion in lung adenocarcinoma via mediating miR-625-5p/CPSF7 axis. Cancer Cell Int 20:24 (2020). PubMed: 31997940
- Davidson L et al. Rapid Depletion of DIS3, EXOSC10, or XRN2 Reveals the Immediate Impact of Exoribonucleolysis on Nuclear RNA Metabolism and Transcriptional Control. Cell Rep 26:2779-2791.e5 (2019). PubMed: 30840897