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Epigenetics and Nuclear Signaling DNA / RNA DNA Synthesis Topoisomerases
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CPT 11 (Irinotecan), DNA topoisomerase I inhibitor (ab141107)

  • Datasheet
  • SDS
  • COA
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Functional Studies - CPT 11 (Irinotecan), DNA topoisomerase I inhibitor (ab141107)

    Key features and details

    • Potent DNA topoisomerase I inhibitor
    • CAS Number: 100286-90-6
    • Purity: > 99%
    • Soluble in water to 25 mM and in DMSO to 100 mM
    • Form / State: Solid
    • Source: Synthetic

    Overview

    • Product name

      CPT 11 (Irinotecan), DNA topoisomerase I inhibitor
    • Description

      Potent DNA topoisomerase I inhibitor
    • Alternative names

      • Irinotecan Hydrochloride
    • Biological description

      Potent DNA topoisomerase I inhibitor. Displays antitumor activity against a variety of tumor types. Active metabolite SN38 (ab141108) also available.

    • Purity

      > 99%
    • CAS Number

      100286-90-6
    • Chemical structure

      Chemical Structure

    Properties

    • Chemical name

      (4S)-4,11-Diethyl-3,4,12,14-tetrahydro-4-hydroxy-3,14-dioxo-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinolin-9-yl [1,4'-bipiperidine]-1'-carboxylic acid ester hydrochloride
    • Molecular weight

      623.15
    • Molecular formula

      C33H38N4O6.HCl
    • PubChem identifier

      74990
    • Storage instructions

      Store at +4°C. The product can be stored for up to 12 months.
    • Solubility overview

      Soluble in water to 25 mM and in DMSO to 100 mM
    • Handling

      Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

      Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

    • SMILES

      Cl.CC[C@]3(O)C=4C=C5c6nc7ccc(OC(=O)N1CCC(CC1)N2CCCCC2)cc7c(CC)c6CN5C(=O)C=4COC3=O
    • Source

      Synthetic

    • Research areas

      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Synthesis
      • Topoisomerases
      • Biochemicals
      • Product Range
      • Just Add Water
      • Biochemicals
      • Chemical Type
      • Biochemicals
      • Biochemicals
      • Pharmacology
      • Enzymes
      • Isomerase
      • Topoisomerase
      • Inhibitors
      • Biochemicals
      • Pharmacology
      • Signaling
      • DNA, RNA & protein synthesis
      • Topoisomerase
      • Biochemicals
      • Research Area
      • Cancer
      • DNA, RNA & protein synthesis
      • Topoisomerase

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab141107 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies
    Use at an assay dependent concentration.
    Notes
    Functional Studies
    Use at an assay dependent concentration.

    Images

    • Functional Studies - CPT 11 (Irinotecan), DNA topoisomerase I inhibitor (ab141107)
      Functional Studies - CPT 11 (Irinotecan), DNA topoisomerase I inhibitor (ab141107)
      ab2893 staining γH2A.X in HeLa cells treated with CPT 11 (Irinotecan) (ab141107), by ICC/IF. Increase of γH2A.X nuclear expression correlates with increased concentration of CPT 11 (Irinotecan), as described in literature.
      The cells were incubated at 37°C for 6 hours in media containing different concentrations of ab141107 (CPT 11 (Irinotecan)) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab2893 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
    • COA
  • References (0)

    Publishing research using ab141107? Please let us know so that we can cite the reference in this datasheet.

    ab141107 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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