Question (68275) | Anti-CPT1A antibody [8F6AE9] (ab128568)

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Question

I've done many mitochondria isolation in liver and it works. So, I just wanted to know if there is a difference in mitochondria isolation in the heart.
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Answer

Hello. I have contacted the laboratory regarding differences between mitchondria isolation in the heart and they have said there is not many differences. Mitochondria preparations are typically better from the heart (purer and higher yield).Heart contains about 30% mitochondria to begin with. Dissection and homogenization are critical because the muscle is not a soft tissue. Buffers are the same. Please let me know if I can be of further assistance.

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