Overview

  • Product name
    Anti-CPT1A antibody [8F6AE9] (HRP)
    See all CPT1A primary antibodies
  • Description
    Mouse monoclonal [8F6AE9] to CPT1A (HRP)
  • Host species
    Mouse
  • Conjugation
    HRP
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Human CPT1A aa 450 to the C-terminus.

  • Positive control
    • WB: HepG2 whole cell lysate. Human heart and rat liver tissue lysates. IHC-P: normal human colon tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab198494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Tissue specificity
    Strong expression in kidney and heart, and lower in liver and skeletal muscle.
  • Pathway
    Lipid metabolism; fatty acid beta-oxidation.
  • Involvement in disease
    Defects in CPT1A are the cause of carnitine palmitoyltransferase 1A deficiency (CPT1AD) [MIM:255120]; also known as CPT-I deficiency or CPT1A deficiency. CPT1AD is a rare autosomal recessive metabolic disorder of long-chain fatty acid oxidation characterized by severe episodes of hypoketotic hypoglycemia usually occurring after fasting or illness. Onset is in infancy or early childhood.
  • Sequence similarities
    Belongs to the carnitine/choline acetyltransferase family.
  • Cellular localization
    Mitochondrion outer membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Carnitine O palmitoyltransferase 1 liver isoform antibody
    • Carnitine O palmitoyltransferase I antibody
    • Carnitine O palmitoyltransferase I liver isoform antibody
    • Carnitine O-palmitoyltransferase 1 antibody
    • Carnitine O-palmitoyltransferase I antibody
    • Carnitine palmitoyltransferase 1A (liver) antibody
    • Carnitine palmitoyltransferase 1A antibody
    • Carnitine palmitoyltransferase I antibody
    • Carnitine palmitoyltransferase I liver antibody
    • CPT 1 antibody
    • CPT I antibody
    • CPT1 antibody
    • CPT1 L antibody
    • CPT1-L antibody
    • Cpt1a antibody
    • CPT1A_HUMAN antibody
    • CPTI antibody
    • CPTI-L antibody
    • L CPT1 antibody
    • liver isoform antibody
    see all

Images

  • IHC image of CPT1A staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab198494 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-CPT1A antibody [8F6AE9] (HRP) (ab198494) at 1/5000 dilution

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : Heart (Human) Tissue Lysate - adult normal tissue
    Lane 3 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 88 kDa
    Observed band size: 88 kDa


    Exposure time: 1 minute


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab198494 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

This product has been referenced in:
  • Li X  et al. Paternal hyperglycemia induces transgenerational inheritance of susceptibility to hepatic steatosis in rats involving altered methylation on Ppara promoter. Biochim Biophys Acta Mol Basis Dis 1865:147-160 (2019). Read more (PubMed: 30404040) »
  • Huang Q  et al. Incremental effect of liraglutide on traditional insulin injections in rats with type 2 diabetes mellitus by maintaining glycolipid metabolism and cardiovascular function. Exp Ther Med 17:1863-1869 (2019). Read more (PubMed: 30783461) »
See all 2 Publications for this product

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