Product nameAnti-CPT1B antibody
See all CPT1B primary antibodies
DescriptionRabbit polyclonal to CPT1B
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Horse, Dog, Pig, Chimpanzee, Macaque monkey
Synthetic peptide corresponding to Human CPT1B aa 200-300 conjugated to keyhole limpet haemocyanin.
Database link: Q92523
- This antibody gave a positive signal in the following tissue lysates: Human Heart; Human Fetal Heart; Human Skeletal Muscle. IHC-P: Human heart FFPE tissue sections.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab134988 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).|
Tissue specificityStrong expression in heart and skeletal muscle. No expression in liver and kidney.
PathwayLipid metabolism; fatty acid beta-oxidation.
Sequence similaritiesBelongs to the carnitine/choline acetyltransferase family.
Cellular localizationMitochondrion outer membrane.
- Information by UniProt
- muscle isoform antibody
- Carnitine O palmitoyltransferase 1B antibody
- Carnitine O palmitoyltransferase I mitochondrial muscle isoform antibody
IHC image of CPT1B staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab134988, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-CPT1B antibody (ab134988) at 1 µg/ml (Milk blocking 3%)
Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue
Lane 2 : Heart (Human) Whole Cell Lysate - fetal normal tissue
Lane 3 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue
Lane 4 : Liver (Human) Tissue Lysate - adult normal tissue (Negative control)
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 87 kDa
Observed band size: 87 kDa
Exposure time: 4 minutes
Please note that Human Liver tissue lysate in Lane 4 was used as a negative control.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab134988 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab134988 has been referenced in 2 publications.
- Yang Y et al. Chinese patent medicine Xin-Ke-Shu inhibits Ca2+ overload and dysfunction of fatty acid ß-oxidation in rats with myocardial infarction induced by LAD ligation. J Chromatogr B Analyt Technol Biomed Life Sci 1079:85-94 (2018). PubMed: 29454926
- Eleftheriadis T et al. Indoleamine 2,3-dioxygenase, by degrading L-tryptophan, enhances carnitine palmitoyltransferase I activity and fatty acid oxidation, and exerts fatty acid-dependent effects in human alloreactive CD4+ T-cells. Int J Mol Med 38:1605-1613 (2016). PubMed: 27667153