Overview

  • Product name
  • Description
    Goat polyclonal to CPT1B
  • Host species
    Goat
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Sheep, Pig
  • Immunogen

    Synthetic peptide: DIADLFQVPKAYS, corresponding to C terminal amino acids 760-772 of Human CPT1B.

Properties

Applications

Our Abpromise guarantee covers the use of ab15703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 - 5 µg/ml.
WB Use at an assay dependent dilution. Can be blocked with Human CPT1B peptide (ab23261). Preliminary experiments gave no signal but low background in human kidney and HepG2 lysates at up to 1µg/ml. We would appreciate any feedback from customers.

Target

  • Tissue specificity
    Strong expression in heart and skeletal muscle. No expression in liver and kidney.
  • Pathway
    Lipid metabolism; fatty acid beta-oxidation.
  • Sequence similarities
    Belongs to the carnitine/choline acetyltransferase family.
  • Cellular localization
    Mitochondrion outer membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • muscle isoform antibody
    • Carnitine O palmitoyltransferase 1B antibody
    • Carnitine O palmitoyltransferase I mitochondrial muscle isoform antibody
    • Carnitine O palmitoyltransferase I muscle isoform antibody
    • Carnitine O-palmitoyltransferase 1, muscle isoform antibody
    • Carnitine O-palmitoyltransferase I antibody
    • Carnitine palmitoyltransferase 1A (muscle) antibody
    • Carnitine palmitoyltransferase 1B (muscle) antibody
    • Carnitine palmitoyltransferase 1B antibody
    • Carnitine palmitoyltransferase I like protein antibody
    • Carnitine palmitoyltransferase I muscle antibody
    • Carnitine palmitoyltransferase I-like protein antibody
    • CPT 1B antibody
    • CPT I antibody
    • CPT1 M antibody
    • CPT1 muscle antibody
    • CPT1-M antibody
    • Cpt1b antibody
    • CPT1B_HUMAN antibody
    • CPT1M antibody
    • CPTI antibody
    • CPTI M antibody
    • CPTI muscle antibody
    • CPTI-M antibody
    • CPTIM antibody
    • FLJ55729 antibody
    • FLJ58750 antibody
    • KIAA1670 antibody
    • M CPT1 antibody
    • M-CPT1 antibody
    • MCCPT1 antibody
    • MCPT1 antibody
    see all

Images

  • ab15703 at 3.8µg/ml staining human heart tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue underwent antigen retrieval by steam in citrate buffer pH 6.0. The AP-staining procedure was used for detection.

References

ab15703 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry. We don't currently have any information about this antibody will work with mouse samples. The immunogen used to generate ab15703 was: a synthetic peptide: DIADLFQVPKAYS (Human) (C terminal), and the antibody is expected to to cross-react with Human (100% identity with immunogen), Pig (90% identity with immunogen) and Sheep (90% identity with immunogen) due to sequence homology. I don't know if you noticed, but ab15703 is a Fast-Track antibody. Fast-Track antibodies are immunogen affinity purified and are shown by ELISA to have high titre values against their immunogen. We are currently testing them for use in Western blotting. At this stage, there can be delays. We sometimes experience problems in searching for / developing the correct lysate or knock-out cell-line. In addition, certain products can be difficult to characterise, due, for instance, to low expression levels or a lack of expression in easily available lysates. For more information regarding this, please see the Fast-Track terms of use located on the online datasheet. So far, ab15703 was tested tested in peptide ELISA at a dilution of 1/16000, and in WB - no signal but low background in Human Kidney and HepG2 lysates at up to 1 µg/ml. If you choose to purchase this antibody, we would greatly appreciate your feedback on these antibodies, whether positive or negative. We will award $300 of Conference Travel Money to the first researcher who sends us positive feedback (including an image and details of materials & methods). Conclusive negative feedback that leads us to withdraw the antibody will also be rewarded with $300 of Conference Travel Money. If you have any more questions, please let us know!

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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