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  1. Link

    cpt2-antibody-epr13626-c-terminal-ab181114.pdf

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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Western blot - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
  • Immunocytochemistry/ Immunofluorescence - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
  • Western blot - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
  • Western blot - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
  • Western blot - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
  • Western blot - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
  • Immunocytochemistry/ Immunofluorescence - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
  • Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13626] to CPT2 - C-terminal
  • Suitable for: IHC-P, WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-CPT2 antibody [EPR13626] - C-terminal
    See all CPT2 primary antibodies
  • Description

    Rabbit monoclonal [EPR13626] to CPT2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1, HeLa, MCF7 whole cell lysates; Mouse heart and kidney tissue lysates; kidney and liver tissue lysates; Human fetal kidney and liver tissue lysates ICC/IF: MCF7 cells IHC-P: Rat colon; Mouse kidney; Human liver carcinoma and skeletal muscle.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13626
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Fatty acids
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-CPT2 antibody [EPR13626] - C-terminal (ab210037)
    • Anti-CPT2 antibody [EPR13626] - BSA and Azide free (ab231162)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human CPT2 knockout HeLa cell line (ab265931)
  • KO cell lysates

    • Human CPT2 knockout HeLa cell lysate (ab257180)
  • Recombinant Protein

    • Recombinant Human CPT2 protein (ab114539)
  • Related Products

    • Recombinant Human CPT2 protein (ab114539)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB
1/1000 - 1/10000. Detects a band of approximately 67 kDa (predicted molecular weight: 74 kDa).
ICC/IF
1/50 - 1/100.
Notes
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB
1/1000 - 1/10000. Detects a band of approximately 67 kDa (predicted molecular weight: 74 kDa).
ICC/IF
1/50 - 1/100.

Target

  • Pathway

    Lipid metabolism; fatty acid beta-oxidation.
  • Involvement in disease

    Defects in CPT2 are the cause of carnitine palmitoyltransferase 2 deficiency (CPT2D) [MIM:255110, 600649]; also known as CPT-II deficiency or CPT2 deficiency. CPT2D is an autosomal recessive disorder characterized by recurrent myoglobinuria, episodes of muscle pain, stiffness, and rhabdomyolysis. These symptoms are triggered by prolonged exercise, fasting or viral infection and patients are usually young adults. In addition to this classical, late-onset, muscular type, a hepatic or hepatocardiomuscular form has been reported in infants. Clinical pictures in these children or neonates include hypoketotic hypoglycemia, liver dysfunction, cardiomyopathy and sudden death.
    Defects in CPT2 are the cause of carnitine palmitoyltransferase 2 deficiency, lethal neonatal (CPT2D-LN) [MIM:608836]; also known as lethal neonatal CPT-II deficiency. It is a lethal neonatal form of CPT2D. This rarely presentation is antenatal with cerebral periventricular cysts and cystic dysplastic kidneys. The clinical variability of the disease is likely attributed to the variable residual enzymatic activity.
  • Sequence similarities

    Belongs to the carnitine/choline acetyltransferase family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Target information above from: UniProt accession P23786 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1376 Human
    • Entrez Gene: 12896 Mouse
    • Entrez Gene: 25413 Rat
    • Omim: 600650 Human
    • SwissProt: P23786 Human
    • SwissProt: P52825 Mouse
    • SwissProt: P18886 Rat
    • Unigene: 713535 Human
    • Unigene: 307620 Mouse
    • Unigene: 11389 Rat
    see all
  • Alternative names

    • Carnitine O palmitoyltransferase 2 antibody
    • Carnitine O palmitoyltransferase 2 mitochondrial antibody
    • Carnitine O-palmitoyltransferase 2 antibody
    • Carnitine palmitoyltransferase 2 antibody
    • Carnitine palmitoyltransferase II antibody
    • CPT 1 antibody
    • CPT 2 antibody
    • CPT II antibody
    • CPT1 antibody
    • CPT2 antibody
    • CPT2_HUMAN antibody
    • CPTASE antibody
    • CPTII antibody
    • IIAE4 antibody
    • mitochondrial antibody
    see all

Images

  • Western blot - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
    Western blot - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
    All lanes : Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : CPT2 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 74 kDa
    Observed band size: 74 kDa



    Lanes 1- 2: Merged signal (red and green). Green - ab181114 observed at 74 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab181114 was shown to react with CPT2/CPT1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265931 (knockout cell lysate ab257180) was used. Wild-type HeLa and CPT2 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab181114 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
    Immunocytochemistry/ Immunofluorescence - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)

    Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling CPT2 with purified ab181114 at 1/100. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab7291, a mouse anti-tubulin antibody (1/1000) using ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) as the secondary. Nuclei couterstained with DAPI (blue).

    For negative control 1, rabbit primary antibody was used, followed by anti-mouse secondary antibody (ab150120). For negative control 2, mouse primary antibody (ab7291) was used followed by anti-rabbit secondary antibody (ab150077).  

  • Western blot - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
    Western blot - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
    All lanes : Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : CPT2 knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : MCF7 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 74 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab181114 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab181114 was shown to specifically react with CPT2 in wild-type HAP1 cells. No band was observed when CPT2 knockout samples were examined. Wild-type and CPT2 knockout samples were subjected to SDS-PAGE.  Ab181114 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
    Western blot - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
    All lanes : Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114) at 1/2000 dilution (purified)

    Lane 1 : Human fetal kidney tissue lysate
    Lane 2 : MCF-7 (human breast carcinoma) whole cell lysate
    Lane 3 : Mouse heart tissue lysate
    Lane 4 : Mouse kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 74 kDa
    Observed band size: 67 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer 5% NFDM/TBST
  • Western blot - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
    Western blot - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
    All lanes : Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114) at 1/1000 dilution (purified)

    Lane 1 : Rat kidney tissue lysate
    Lane 2 : Rat liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 74 kDa
    Observed band size: 67 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer 5% NFDM/TBST
  • Western blot - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
    Western blot - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
    All lanes : Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114) at 1/10000 dilution (unpurified)

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : Human fetal kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 74 kDa
    Observed band size: 67 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)

    Immunohistochemical analysis of paraffin embedded rat colon tissue section labelling CPT2 with purified ab181114 at dilution of 1/50. The secondary antibody used was HRP-conjugated Goat Anti-Rabbit IgG H&L (ab97051) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)

    Immunohistochemical analysis of paraffin embedded mouse kidney tissue section labelling CPT2 with purified ab181114 at dilution of 1/50. The secondary antibody used was HRP-conjugated Goat Anti-Rabbit IgG H&L (ab97051) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)

    Immunohistochemical analysis of paraffin embedded human liver carcinoma tissue section labelling CPT2 with purified ab181114 at dilution of 1/50. The secondary antibody used was Goat Anti-Rabbit IgG H&L (HRP) (ab97051), at a dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CPT2 with unpurified ab181114 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling CPT2 with unpurified ab181114 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
    Immunocytochemistry/ Immunofluorescence - Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling CPT2 with unpurified ab181114 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi.

  • Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)
    Anti-CPT2 antibody [EPR13626] - C-terminal (ab181114)

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (16)

Publishing research using ab181114? Please let us know so that we can cite the reference in this datasheet.

ab181114 has been referenced in 16 publications.

  • Guo X  et al. HRD1 inhibits fatty acid oxidation and tumorigenesis by ubiquitinating CPT2 in triple-negative breast cancer. Mol Oncol 15:642-656 (2021). PubMed: 33207079
  • Huang YW  et al. Dysregulated Free Fatty Acid Receptor 2 Exacerbates Colonic Adenoma Formation in ApcMin/+ Mice: Relation to Metabolism and Gut Microbiota Composition. J Cancer Prev 26:32-40 (2021). PubMed: 33842404
  • Sun C  et al. Mass spectrometry imaging-based metabolomics to visualize the spatially resolved reprogramming of carnitine metabolism in breast cancer. Theranostics 10:7070-7082 (2020). PubMed: 32641979
  • Zhang J  et al. Molecular Profiling Reveals a Common Metabolic Signature of Tissue Fibrosis. Cell Rep Med 1:100056 (2020). PubMed: 33205063
  • Ferchaud-Roucher V  et al. Maternal obesity results in decreased syncytiotrophoblast synthesis of palmitoleic acid, a fatty acid with anti-inflammatory and insulin-sensitizing properties. FASEB J 33:6643-6654 (2019). PubMed: 30811959
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Immunohistochemistry (Frozen sections) abreview for Anti-CPT2/CPT1 antibody [EPR13626] - C-terminal

Below Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain-Hippocampus)
Permeabilization
Yes - .1%TX-100
Specification
Brain-Hippocampus
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10%
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 24 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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