Key features and details
- Rabbit polyclonal to CRABP2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-CRABP2 antibody
See all CRABP2 primary antibodies
DescriptionRabbit polyclonal to CRABP2
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig, Rhesus monkey
Recombinant fragment within Human CRABP2 (internal sequence). The exact sequence is proprietary.
Database link: P29373
- WB: Mouse brain and fetal brain lysates; Rat brain lysate; HepG2 whole cell lysate. IHC-P: Adult mouse retina tissue; MDAMB468 xenograft tissue. ICC/IF: HCT 116 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 16 kDa.|
FunctionTransports retinoic acid to the nucleus. Regulates the access of retinoic acid to the nuclear retinoic acid receptors.
Sequence similaritiesBelongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
DomainForms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
Cellular localizationCytoplasm. Nucleus. Upon ligand binding, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus.
- Information by UniProt
- Cellular retinoic acid binding protein 2 antibody
- Cellular retinoic acid binding protein II antibody
- Cellular retinoic acid-binding protein 2 antibody
All lanes : Anti-CRABP2 antibody (ab227020) at 1/1000 dilution
Lane 1 : Mouse brain extract
Lane 2 : Mouse fetal brain extract
Lane 3 : Rat brain extract
Lysates/proteins at 50 µg per lane.
Predicted band size: 16 kDa
15% SDS-PAGE gel.
Paraffin-embedded adult mouse retina tissue stained for CRABP2 using ab227020 at 1/250 dilution (green) in immunohistochemical analysis.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 [GT11710] antibody diluted at 1/500 dilution.
HCT 116 (human colorectal carcinoma cell line) cells stained for CRABP2 (green) using ab227020 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde for 15 minutes at RT.
Blue: Hoechst 33342 staining.
Anti-CRABP2 antibody (ab227020) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 16 kDa
15% SDS PAGE gel.
Paraffin-embedded MDAMB468 xenograft tissue stained for CRABP2 using ab227020 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227020 has not yet been referenced specifically in any publications.