Overview

  • Product name
  • Description
    Rabbit polyclonal to CRABP2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human CRABP2 (internal sequence). The exact sequence is proprietary.
    Database link: P29373

  • Positive control
    • WB: Mouse brain and fetal brain lysates; Rat brain lysate; HepG2 whole cell lysate. IHC-P: Adult mouse retina tissue; MDAMB468 xenograft tissue. ICC/IF: HCT 116 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 16 kDa.

Target

Images

  • All lanes : Anti-CRABP2 antibody (ab227020) at 1/1000 dilution

    Lane 1 : Mouse brain extract
    Lane 2 : Mouse fetal brain extract
    Lane 3 : Rat brain extract

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 16 kDa



    15% SDS-PAGE gel.

  • Paraffin-embedded adult mouse retina tissue stained for CRABP2 using ab227020 at 1/250 dilution (green) in immunohistochemical analysis.

    Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 [GT11710] antibody diluted at 1/500 dilution.
    Blue: DAPI.

  • HCT 116 (human colorectal carcinoma cell line) cells stained for CRABP2 (green) using ab227020 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde for 15 minutes at RT.

    Blue: Hoechst 33342 staining.

  • Anti-CRABP2 antibody (ab227020) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

    Predicted band size: 16 kDa



    15% SDS PAGE gel.

  • Paraffin-embedded MDAMB468 xenograft tissue stained for CRABP2 using ab227020 at 1/500 dilution in immunohistochemical analysis.

References

ab227020 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227020.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up