Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)

Submit a review Submit a question
Western blot - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
  • Western blot - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
  • Immunocytochemistry/ Immunofluorescence - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
  • Flow Cytometry (Intracellular) - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
  • Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14256(B)] to CRABP2 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free
    See all CRABP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR14256(B)] to CRABP2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250468 is the carrier-free version of ab181255.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab250468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).

Target

  • Function

    Transports retinoic acid to the nucleus. Regulates the access of retinoic acid to the nuclear retinoic acid receptors.

  • Sequence similarities

    Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

  • Domain

    Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.

  • Cellular localization

    Cytoplasm. Nucleus. Upon ligand binding, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Cellular retinoic acid binding protein 2 antibody
      • Cellular retinoic acid binding protein II antibody
      • Cellular retinoic acid-binding protein 2 antibody
      • Cellular retinoic acid-binding protein II antibody
      • CRABP II antibody
      • CRABP-II antibody
      • Crabp2 antibody
      • CRABPII antibody
      • RABP2_HUMAN antibody
      • RBP6 antibody
      • Rretinoic acid-binding protein, cellular, type II antibody
      see all

    Images

    • Western blot - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
      Western blot - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
      Anti-CRABP2 antibody [EPR14256(B)] (ab181255) at 1/50000 dilution + MCF7 cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 16 kDa
      Observed band size: 16 kDa



      This data was developed using ab181255, the same antibody clone in a different buffer formulation.

    • Western blot - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
      Western blot - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
      Anti-CRABP2 antibody [EPR14256(B)] (ab181255) at 1/10000 dilution + Human skin tissue lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

      Developed using the ECL technique.

      Predicted band size: 16 kDa
      Observed band size: 16 kDa



      This data was developed using ab181255, the same antibody clone in a different buffer formulation.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
      This data was developed using ab181255, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded, Human liver tissue labeling CRABP2 with ab181255 at a 1/100 dilution. Counter stained with hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
      This data was developed using ab181255, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded, Human kidney tissue labeling CRABP2 with ab181255 at a 1/100 dilution. Counter stained with hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    • Immunocytochemistry/ Immunofluorescence - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
      Immunocytochemistry/ Immunofluorescence - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
      This data was developed using ab181255, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of, paraformaldehyde-fixed, MCF7 cells labeling CRABP2 with ab181255 at a 1/250 dilution. As secondary antibody Goat anti rabbit IgG (Alexa Fluor®488) (green) was used at a 1/200 dilution. DAPI staining in blue.
    • Flow Cytometry (Intracellular) - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
      Flow Cytometry (Intracellular) - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)

      This data was developed using ab181255, the same antibody clone in a different buffer formulation. 

      Intracellular Flow Cytometry analysis of, paraformaldehyde-fixed, MCF7 cells labeling CRABP2 (red) with ab181255 at a 1/80 dilution, or negative control rabbit monoclonal IgG (green). As secondary antibody goat anti rabbit IgG (FITC) was used at a 1/150 dilution. 

       

       

    • Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
      Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab250468? Please let us know so that we can cite the reference in this datasheet.

    ab250468 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab250468.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com