Name: Anti-CRABP2 antibody [EPR17376]
Brand: RabMAb
SKU: ab211927

Add a compatible secondary antibody

Primary

Secondary

This product Rabbit Anti-CRABP2 antibody [EPR17376] (ab211927)
ICC/IF, Flow Cyt, IHC-P, WB

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
ICC/IF, Flow Cyt, IHC-P, ELISA, IHC-Fr

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Product name

Anti-CRABP2 antibody [EPR17376]
See all CRABP2 primary antibodies
Description

Rabbit monoclonal [EPR17376] to CRABP2
Host species

Rabbit
Tested applications

Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant full length protein within Human CRABP2 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P29373

Run BLAST with Run BLAST with
Positive control
- WB: Human, mouse and rat skin lysates; HT-29 and MCF7 whole cell lysates. IHC-P: Human oesophagus, skin and pancreatic ductal adenocarcinoma tissues; mouse and rat skin tissues. ICC/IF: MCF7 and HT-29 cells. Flow Cyt: MCF7 cells.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17376
Isotype

IgG
Research areas

Alternative Versions
- Anti-CRABP2 antibody [EPR17376] - BSA and Azide free (ab223551)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human CRABP2 protein (ab74527)

Our Abpromise guarantee covers the use of ab211927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ICC/IF		1/250.
Flow Cyt		1/600. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P		1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB		1/1000. Detects a band of approximately 14 kDa (predicted molecular weight: 16 kDa).

Function

Transports retinoic acid to the nucleus. Regulates the access of retinoic acid to the nuclear retinoic acid receptors.
Sequence similarities

Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
Domain

Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
Cellular localization

Cytoplasm. Nucleus. Upon ligand binding, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus.
Target information above from: UniProt accession P29373 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 1382 Human
- Entrez Gene: 12904 Mouse
- Entrez Gene: 29563 Rat
- Omim: 180231 Human
- SwissProt: P29373 Human
- SwissProt: P22935 Mouse
- SwissProt: P51673 Rat
- Unigene: 405662 Human
- Unigene: 4757 Mouse
- Unigene: 11333 Rat
see all
Alternative names
- Cellular retinoic acid binding protein 2 antibody
- Cellular retinoic acid binding protein II antibody
- Cellular retinoic acid-binding protein 2 antibody
- Cellular retinoic acid-binding protein II antibody
- CRABP II antibody
- CRABP-II antibody
- Crabp2 antibody
- CRABPII antibody
- RABP2_HUMAN antibody
- RBP6 antibody
- Rretinoic acid-binding protein, cellular, type II antibody
see all

Western blot - Anti-CRABP2 antibody [EPR17376] (ab211927)

Lanes 1 & 3 : Anti-CRABP2 antibody [EPR17376] (ab211927) at 1/5000 dilution
Lane 2 : Anti-CRABP2 antibody [EPR17376] (ab211927) at 1/1000 dilution

Lane 1 : Human skin lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
Lane 1 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

Predicted band size: 16 kDa
Observed band size: 14 kDa
why is the actual band size different from the predicted?

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-CRABP2 antibody [EPR17376] (ab211927)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling CRABP2 with ab211927 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary at 1/1000 dilution (green). Confocal image showing nuclear and cytoplasmic staining on MCF7 cell line. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows:
-ve control 1: ab211927 at 1/250 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed, by followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR17376] (ab211927)

Immunohistochemical analysis of paraffin-embedded human oesophagus tissue labeling CRABP2 with ab211927 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and nuclear staining on the stratified squamous epithelium of the human oesophagus is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Western blot - Anti-CRABP2 antibody [EPR17376] (ab211927)

All lanes : Anti-CRABP2 antibody [EPR17376] (ab211927) at 1/1000 dilution

Lane 1 : Mouse skin lysate
Lane 2 : Rat skin lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 16 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR17376] (ab211927)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling CRABP2 with ab211927 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and nuclear staining on the stratified squamous epithelium and hair follicle cells of the human skin is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR17376] (ab211927)

Immunohistochemical analysis of paraffin-embedded human pancreatic ductal adenocarcinoma tissue labeling CRABP2 with ab211927 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and nuclear staining on the human pancreatic ductal adenocarcinoma is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR17376] (ab211927)

Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling CRABP2 with ab211927 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and nuclear staining on the stratified squamous epithelium, hair follicle cells and sweat gland cells of the mouse skin is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR17376] (ab211927)

Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling CRABP2 with ab211927 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and nuclear staining on the stratified squamous epithelium and sweat gland cells of the rat skin is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-CRABP2 antibody [EPR17376] (ab211927)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (Human colorectal adenocarcinoma cell line) cells labeling CRABP2 with ab211927 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and cytoplasmic staining on HT-29 cell line. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows:
-ve control 1: ab211927 at 1/250 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Flow Cytometry - Anti-CRABP2 antibody [EPR17376] (ab211927)

Flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling CRABP2 with ab211927 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Datasheet

ab211927 has not yet been referenced specifically in any publications.

Publishing research using ab211927? Please let us know so that we can cite the reference in this datasheet.

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Recombinant Anti-CRABP2 antibody [EPR17376] (ab211927)

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