Product nameAnti-CRALBP antibody [B2]
See all CRALBP primary antibodies
DescriptionMouse monoclonal [B2] to CRALBP
Tested applicationsSuitable for: ICC/IF, WB, IHC-Fr, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Cow, Human, Pig, Monkey
Recombinant full length protein (Human).
- This antibody gave a positive signal in the following tissue lysates: rat eye; rat retina.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
Light chain typekappa
Our Abpromise guarantee covers the use of ab15051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 22892561|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa).|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionSoluble retinoid carrier essential the proper function of both rod and cone photoreceptors. Participates in the regeneration of active 11-cis-retinol and 11-cis-retinaldehyde, from the inactive 11-trans products of the rhodopsin photocycle and in the de novo synthesis of these retinoids from 11-trans metabolic precursors. The cycling of retinoids between photoreceptor and adjacent pigment epithelium cells is known as the 'visual cycle'.
Tissue specificityRetina and pineal gland. Not present in photoreceptor cells but is expressed abundantly in the adjacent retinal pigment epithelium (RPE) and in the Mueller glial cells of the retina.
Involvement in diseaseDefects in RLBP1 are a cause of retinitis pigmentosa autosomal recessive (ARRP) [MIM:268000]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
Defects in RLBP1 are the cause of Bothnia retinal dystrophy (BRD) [MIM:607475]; also known as Vasterbotten dystrophy. Affected individuals show night blindness from early childhood with features consistent with retinitis punctata albescens and macular degeneration.
Defects in RLBP1 are the cause of rod-cone dystrophy Newfoundland (NFRCD) [MIM:607476]. NFRCD is a retinal dystrophy reminiscent of retinitis punctata albescens but with a substantially lower age at onset and more-rapid and distinctive progression. Rod-cone dystrophies results from initial loss of rod photoreceptors, later followed by cone photoreceptors loss.
Defects in RLBP1 are a cause of fundus albipunctatus (FA) [MIM:136880]. FA is a rare form of stationary night blindness characterized by a delay in the regeneration of cone and rod photopigments.
Sequence similaritiesContains 1 CRAL-TRIO domain.
- Information by UniProt
- Cellular retinaldehyde binding protein 1 antibody
- Cellular retinaldehyde binding protein antibody
- Cellular retinaldehyde-binding protein antibody
All lanes : Anti-CRALBP antibody [B2] (ab15051) at 1 µg/ml
Lane 1 :
Rat eye tissue lysate - total protein (ab4035)
Lane 2 : Rat retina tissue lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Immunofluorescence analysis of hESC-RPE monolayers, staining CRALBP (green) with ab15051.
Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 1 hour. Cells were incubated with primary antibody (1/1000) for 1 hour at room temperature. An AlexaFluor®488-conjugated donkey anti-mouse IgG (1/800) was used as the secondary antibody.
ab15051 staining rat retina sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab15051 at 1/250 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-mouse polyclonal antibody at 1/250 was used as the secondary antibody. Müller cells in the eye retina are positive. Two different areas of the retina are shown.
This product has been referenced in:
- Shrestha R et al. Aberrant hiPSCs-Derived from Human Keratinocytes Differentiates into 3D Retinal Organoids that Acquire Mature Photoreceptors. Cells 8:N/A (2019). Read more (PubMed: 30634512) »
- You Y et al. Evidence of Müller Glial Dysfunction in Patients with Aquaporin-4 Immunoglobulin G-Positive Neuromyelitis Optica Spectrum Disorder. Ophthalmology N/A:N/A (2019). Read more (PubMed: 30711604) »