Key features and details
- Mouse monoclonal [B2] to CRALBP
- Suitable for: WB
- Reacts with: Mouse, Rat, Cow, Human, Monkey
- Isotype: IgG1
Product nameAnti-CRALBP antibody [B2]
See all CRALBP primary antibodies
DescriptionMouse monoclonal [B2] to CRALBP
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Cow, Human, Monkey
Predicted to work with: Pig
Recombinant full length protein corresponding to Human CRALBP.
Database link: P12271
- This antibody gave a positive signal in the following tissue lysates: rat eye; rat retina.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab15051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa).|
FunctionSoluble retinoid carrier essential the proper function of both rod and cone photoreceptors. Participates in the regeneration of active 11-cis-retinol and 11-cis-retinaldehyde, from the inactive 11-trans products of the rhodopsin photocycle and in the de novo synthesis of these retinoids from 11-trans metabolic precursors. The cycling of retinoids between photoreceptor and adjacent pigment epithelium cells is known as the 'visual cycle'.
Tissue specificityRetina and pineal gland. Not present in photoreceptor cells but is expressed abundantly in the adjacent retinal pigment epithelium (RPE) and in the Mueller glial cells of the retina.
Involvement in diseaseDefects in RLBP1 are a cause of retinitis pigmentosa autosomal recessive (ARRP) [MIM:268000]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
Defects in RLBP1 are the cause of Bothnia retinal dystrophy (BRD) [MIM:607475]; also known as Vasterbotten dystrophy. Affected individuals show night blindness from early childhood with features consistent with retinitis punctata albescens and macular degeneration.
Defects in RLBP1 are the cause of rod-cone dystrophy Newfoundland (NFRCD) [MIM:607476]. NFRCD is a retinal dystrophy reminiscent of retinitis punctata albescens but with a substantially lower age at onset and more-rapid and distinctive progression. Rod-cone dystrophies results from initial loss of rod photoreceptors, later followed by cone photoreceptors loss.
Defects in RLBP1 are a cause of fundus albipunctatus (FA) [MIM:136880]. FA is a rare form of stationary night blindness characterized by a delay in the regeneration of cone and rod photopigments.
Sequence similaritiesContains 1 CRAL-TRIO domain.
- Information by UniProt
- Cellular retinaldehyde binding protein 1 antibody
- Cellular retinaldehyde binding protein antibody
- Cellular retinaldehyde-binding protein antibody
All lanes : Anti-CRALBP antibody [B2] (ab15051) at 1 µg/ml
Lane 1 :
Rat eye tissue lysate - total protein (ab4035)
Lane 2 : Rat retina tissue lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
ab15051 has been referenced in 62 publications.
- Plaza Reyes A et al. Identification of cell surface markers and establishment of monolayer differentiation to retinal pigment epithelial cells. Nat Commun 11:1609 (2020). PubMed: 32231223
- Shrestha R et al. Aberrant hiPSCs-Derived from Human Keratinocytes Differentiates into 3D Retinal Organoids that Acquire Mature Photoreceptors. Cells 8:N/A (2019). PubMed: 30634512
- You Y et al. Evidence of Müller Glial Dysfunction in Patients with Aquaporin-4 Immunoglobulin G-Positive Neuromyelitis Optica Spectrum Disorder. Ophthalmology N/A:N/A (2019). PubMed: 30711604
- Cora V et al. A Cleared View on Retinal Organoids. Cells 8:N/A (2019). PubMed: 31035373
- Shen H et al. A novel xeno-free culture system for human retinal pigment epithelium cells. Int J Ophthalmol 12:563-570 (2019). PubMed: 31024807