Anti-CRALBP antibody [B2] - BSA and Azide free (ab264522)
Key features and details
- Mouse monoclonal [B2] to CRALBP - BSA and Azide free
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG1
Overview
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Product name
Anti-CRALBP antibody [B2] - BSA and Azide free
See all CRALBP primary antibodies -
Description
Mouse monoclonal [B2] to CRALBP - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
Application Species WB Rat -
Immunogen
Recombinant full length protein corresponding to Human CRALBP.
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Positive control
- This antibody gave a positive signal in the following tissue lysates: rat eye; rat retina.
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General notes
ab264522 is the carrier-free version of ab15051.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
B2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab264522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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WB |
Rat
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All applications |
Mouse
Cow
Human
Monkey
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Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa). |
Target
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Function
Soluble retinoid carrier essential the proper function of both rod and cone photoreceptors. Participates in the regeneration of active 11-cis-retinol and 11-cis-retinaldehyde, from the inactive 11-trans products of the rhodopsin photocycle and in the de novo synthesis of these retinoids from 11-trans metabolic precursors. The cycling of retinoids between photoreceptor and adjacent pigment epithelium cells is known as the 'visual cycle'. -
Tissue specificity
Retina and pineal gland. Not present in photoreceptor cells but is expressed abundantly in the adjacent retinal pigment epithelium (RPE) and in the Mueller glial cells of the retina. -
Involvement in disease
Defects in RLBP1 are a cause of retinitis pigmentosa autosomal recessive (ARRP) [MIM:268000]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
Defects in RLBP1 are the cause of Bothnia retinal dystrophy (BRD) [MIM:607475]; also known as Vasterbotten dystrophy. Affected individuals show night blindness from early childhood with features consistent with retinitis punctata albescens and macular degeneration.
Defects in RLBP1 are the cause of rod-cone dystrophy Newfoundland (NFRCD) [MIM:607476]. NFRCD is a retinal dystrophy reminiscent of retinitis punctata albescens but with a substantially lower age at onset and more-rapid and distinctive progression. Rod-cone dystrophies results from initial loss of rod photoreceptors, later followed by cone photoreceptors loss.
Defects in RLBP1 are a cause of fundus albipunctatus (FA) [MIM:136880]. FA is a rare form of stationary night blindness characterized by a delay in the regeneration of cone and rod photopigments. -
Sequence similarities
Contains 1 CRAL-TRIO domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 282038 Cow
- Entrez Gene: 6017 Human
- Entrez Gene: 19771 Mouse
- Entrez Gene: 293049 Rat
- Omim: 180090 Human
- SwissProt: P10123 Cow
- SwissProt: P12271 Human
- SwissProt: Q9Z275 Mouse
see all -
Alternative names
- Cellular retinaldehyde binding protein 1 antibody
- Cellular retinaldehyde binding protein antibody
- Cellular retinaldehyde-binding protein antibody
see all
Images
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All lanes : Anti-CRALBP antibody [B2] (ab15051) at 1 µg/ml
Lane 1 : ab4035, Rat eye tissue lysate, 10 ug
Lane 2 : Rat retina tissue lysate, 10 ug
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Exposure time: 30 secondsThis data was developed using the same antibody clone in a different buffer formulation containing PBS, L-Arginine and sodium azide (ab15051)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab264522 has not yet been referenced specifically in any publications.