Anti-CRALBP antibody [B2] (HRP) (ab199992)

Mouse monoclonal CRALBP antibody [B2] conjugated to HRP. Validated in WB and tested in Rat. Immunogen corresponding to recombinant full length protein.

Overview

  • Product name

    Anti-CRALBP antibody [B2] (HRP)
    See all CRALBP primary antibodies
  • Description

    Mouse monoclonal [B2] to CRALBP (HRP)
  • Host species

    Mouse
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Cow, Human, Pig, Monkey
  • Immunogen

    Recombinant full length protein corresponding to Human CRALBP.

  • Positive control

    • WB: Rat eye lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Proclin
    Constituents: PBS, 1% BSA, 30% Glycerol
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Monoclonal
  • Clone number

    B2
  • Isotype

    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab199992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).

Target

  • Function

    Soluble retinoid carrier essential the proper function of both rod and cone photoreceptors. Participates in the regeneration of active 11-cis-retinol and 11-cis-retinaldehyde, from the inactive 11-trans products of the rhodopsin photocycle and in the de novo synthesis of these retinoids from 11-trans metabolic precursors. The cycling of retinoids between photoreceptor and adjacent pigment epithelium cells is known as the 'visual cycle'.
  • Tissue specificity

    Retina and pineal gland. Not present in photoreceptor cells but is expressed abundantly in the adjacent retinal pigment epithelium (RPE) and in the Mueller glial cells of the retina.
  • Involvement in disease

    Defects in RLBP1 are a cause of retinitis pigmentosa autosomal recessive (ARRP) [MIM:268000]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
    Defects in RLBP1 are the cause of Bothnia retinal dystrophy (BRD) [MIM:607475]; also known as Vasterbotten dystrophy. Affected individuals show night blindness from early childhood with features consistent with retinitis punctata albescens and macular degeneration.
    Defects in RLBP1 are the cause of rod-cone dystrophy Newfoundland (NFRCD) [MIM:607476]. NFRCD is a retinal dystrophy reminiscent of retinitis punctata albescens but with a substantially lower age at onset and more-rapid and distinctive progression. Rod-cone dystrophies results from initial loss of rod photoreceptors, later followed by cone photoreceptors loss.
    Defects in RLBP1 are a cause of fundus albipunctatus (FA) [MIM:136880]. FA is a rare form of stationary night blindness characterized by a delay in the regeneration of cone and rod photopigments.
  • Sequence similarities

    Contains 1 CRAL-TRIO domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cellular retinaldehyde binding protein 1 antibody
    • Cellular retinaldehyde binding protein antibody
    • Cellular retinaldehyde-binding protein antibody
    • MGC3663 antibody
    • Retinaldehyde binding protein 1 antibody
    • Retinaldehyde-binding protein 1 antibody
    • RLBP 1 antibody
    • RLBP1 antibody
    • RLBP1_HUMAN antibody
    see all

Images

  • Anti-CRALBP antibody [B2] (HRP) (ab199992) at 1/5000 dilution + Eye (Rat) Tissue Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 36 kDa
    Observed band size: 36 kDa


    Exposure time: 3 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab199992 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab199992 has not yet been referenced specifically in any publications.

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