Recombinant
RabMAb

Recombinant Anti-CRALBP antibody [EPR13801] (ab183728)

Overview

  • Product name

    Anti-CRALBP antibody [EPR13801]
    See all CRALBP primary antibodies
  • Description

    Rabbit monoclonal [EPR13801] to CRALBP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human CRALBP aa 1-100. The exact sequence is proprietary.
    Database link: P12271

  • Positive control

    • IHC-Fr: rat and mouse retina WB: mouse and rat eyeball lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab183728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/250.
WB 1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IP 1/40 - 1/60.

Target

  • Function

    Soluble retinoid carrier essential the proper function of both rod and cone photoreceptors. Participates in the regeneration of active 11-cis-retinol and 11-cis-retinaldehyde, from the inactive 11-trans products of the rhodopsin photocycle and in the de novo synthesis of these retinoids from 11-trans metabolic precursors. The cycling of retinoids between photoreceptor and adjacent pigment epithelium cells is known as the 'visual cycle'.
  • Tissue specificity

    Retina and pineal gland. Not present in photoreceptor cells but is expressed abundantly in the adjacent retinal pigment epithelium (RPE) and in the Mueller glial cells of the retina.
  • Involvement in disease

    Defects in RLBP1 are a cause of retinitis pigmentosa autosomal recessive (ARRP) [MIM:268000]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
    Defects in RLBP1 are the cause of Bothnia retinal dystrophy (BRD) [MIM:607475]; also known as Vasterbotten dystrophy. Affected individuals show night blindness from early childhood with features consistent with retinitis punctata albescens and macular degeneration.
    Defects in RLBP1 are the cause of rod-cone dystrophy Newfoundland (NFRCD) [MIM:607476]. NFRCD is a retinal dystrophy reminiscent of retinitis punctata albescens but with a substantially lower age at onset and more-rapid and distinctive progression. Rod-cone dystrophies results from initial loss of rod photoreceptors, later followed by cone photoreceptors loss.
    Defects in RLBP1 are a cause of fundus albipunctatus (FA) [MIM:136880]. FA is a rare form of stationary night blindness characterized by a delay in the regeneration of cone and rod photopigments.
  • Sequence similarities

    Contains 1 CRAL-TRIO domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cellular retinaldehyde binding protein 1 antibody
    • Cellular retinaldehyde binding protein antibody
    • Cellular retinaldehyde-binding protein antibody
    • MGC3663 antibody
    • Retinaldehyde binding protein 1 antibody
    • Retinaldehyde-binding protein 1 antibody
    • RLBP 1 antibody
    • RLBP1 antibody
    • RLBP1_HUMAN antibody
    see all

Images

  • Immunohistochemistry (Frozn sections) analysis of rat retina tissue sections labeling CRALBP with purified ab183728 at 1:250 (0.47µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor© 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

  • Immunohistochemistry (Frozn sections) analysis of mouse retina tissue sections labeling CRALBP with purified ab183728 at 1:250 (0.47µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor© 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

  • All lanes : Anti-CRALBP antibody [EPR13801] (ab183728) at 1/5000 dilution

    Lane 1 : mouse eyeball lysate
    Lane 2 : rat eyeball lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 36 kDa

  • Western blot analysis of immunoprecipitation pellet from mouse eyeball lysate immunoprecipitated using ab183728 at 1/50 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

References

ab183728 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2)
Gel Running Conditions
Reduced Denaturing (4-12% BisTris Gel)
Loading amount
20 µg
Specification
HepG2
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Lei Wang

Verified customer

Submitted May 21 2019

Application
Western blot
Sample
Mouse Tissue lysate - whole (retina, eye ball)
Gel Running Conditions
Reduced Denaturing (4-12% BisTris Gel)
Loading amount
15 µg
Specification
retina, eye ball
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted May 20 2019

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