Recombinant
RabMAb

Recombinant Anti-CRALBP antibody [EPR23448-119] (ab243664)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23448-119] to CRALBP
  • Suitable for: IHC-P, IP, WB, IHC-Fr, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CRALBP antibody [EPR23448-119]
    See all CRALBP primary antibodies

  • Description

    Rabbit monoclonal [EPR23448-119] to CRALBP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IP, WB, IHC-Fr, Flow Cytmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human CRALBP aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P12271

  • Positive control

    • WB: Human eyeball tissue lysate; Mouse eyeball tissue and P20 retina lysates; Rat eyeball tissue lysate; SK-MEL-2 and SK-MEL-28 whole cell lysates. IHC-P: Rat retina tissue, Mouse retina tissue; Human retina tissue. IHC-Fr: Mouse retina tissue; Rat retina tissue. Flow Cyt: SK-MEL-28 cells. IP: SK-MEL-28 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23448-119

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab243664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/30.
WB 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IHC-Fr 1/250.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Flow Cyt 1/700.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Function

      Soluble retinoid carrier essential the proper function of both rod and cone photoreceptors. Participates in the regeneration of active 11-cis-retinol and 11-cis-retinaldehyde, from the inactive 11-trans products of the rhodopsin photocycle and in the de novo synthesis of these retinoids from 11-trans metabolic precursors. The cycling of retinoids between photoreceptor and adjacent pigment epithelium cells is known as the 'visual cycle'.

    • Tissue specificity

      Retina and pineal gland. Not present in photoreceptor cells but is expressed abundantly in the adjacent retinal pigment epithelium (RPE) and in the Mueller glial cells of the retina.

    • Involvement in disease

      Defects in RLBP1 are a cause of retinitis pigmentosa autosomal recessive (ARRP) [MIM:268000]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
      Defects in RLBP1 are the cause of Bothnia retinal dystrophy (BRD) [MIM:607475]; also known as Vasterbotten dystrophy. Affected individuals show night blindness from early childhood with features consistent with retinitis punctata albescens and macular degeneration.
      Defects in RLBP1 are the cause of rod-cone dystrophy Newfoundland (NFRCD) [MIM:607476]. NFRCD is a retinal dystrophy reminiscent of retinitis punctata albescens but with a substantially lower age at onset and more-rapid and distinctive progression. Rod-cone dystrophies results from initial loss of rod photoreceptors, later followed by cone photoreceptors loss.
      Defects in RLBP1 are a cause of fundus albipunctatus (FA) [MIM:136880]. FA is a rare form of stationary night blindness characterized by a delay in the regeneration of cone and rod photopigments.

    • Sequence similarities

      Contains 1 CRAL-TRIO domain.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      • Alternative names

        • Cellular retinaldehyde binding protein 1 antibody
        • Cellular retinaldehyde binding protein antibody
        • Cellular retinaldehyde-binding protein antibody
        • MGC3663 antibody
        • Retinaldehyde binding protein 1 antibody
        • Retinaldehyde-binding protein 1 antibody
        • RLBP 1 antibody
        • RLBP1 antibody
        • RLBP1_HUMAN antibody
        see all

      Images

      • Western blot - Anti-CRALBP antibody [EPR23448-119] (ab243664)
        Western blot - Anti-CRALBP antibody [EPR23448-119] (ab243664)
        All lanes : Anti-CRALBP antibody [EPR23448-119] (ab243664) at 1/1000 dilution

        Lane 1 : SK-MEL-2 (human skin malignant melanoma), whole cell lysate
        Lane 2 : SK-MEL-28 (human malignant melanoma), whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

        Predicted band size: 36 kDa
        Observed band size: 36 kDa



        Blocking and diluting buffer and concentration: 5% NFDM/TBST.

        The molecular weight observed is consistent with what has been described in the literature (PMID: 29063836).

        Exposure time: 15 seconds.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] (ab243664)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] (ab243664)

        Immunohistochemical analysis of paraffin-embedded Human retina tissue labeling CRALBP with ab243664 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human retina. The section was incubated with ab243664 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      • Flow Cytometry - Anti-CRALBP antibody [EPR23448-119] (ab243664)
        Flow Cytometry - Anti-CRALBP antibody [EPR23448-119] (ab243664)

        Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SK-MEL-28 (Human malignant melanoma) cells labelling CRALBP with ab243664 at 1/700 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      • Immunoprecipitation - Anti-CRALBP antibody [EPR23448-119] (ab243664)
        Immunoprecipitation - Anti-CRALBP antibody [EPR23448-119] (ab243664)

        CRALBP was immunoprecipitated from 0.35 mg SK-MEL-28 (human malignant melanoma), whole cell lysate with ab243664 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab243664 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

        Lane 1: SK-MEL-28 (human malignant melanoma), whole cell lysate 10 ug

        Lane 2: ab243664 IP in SK-MEL-28 whole cell lysate

        Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab243664 in SK-MEL-28 whole cell lysate

        Blocking and dilution buffer and concentration: 5% NFDM/TBST.

        Exposure time: 100 seconds

         

      • Western blot - Anti-CRALBP antibody [EPR23448-119] (ab243664)
        Western blot - Anti-CRALBP antibody [EPR23448-119] (ab243664)
        All lanes : Anti-CRALBP antibody [EPR23448-119] (ab243664) at 1/1000 dilution

        Lane 1 : Mouse eyeball tissue lysate
        Lane 2 : Rat eyeball tissue lysate
        Lane 3 : Mouse P20 retina tissue lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

        Predicted band size: 36 kDa
        Observed band size: 36 kDa



        Blocking and diluting buffer and concentration: 5% NFDM/TBST.

        The molecular weight observed is consistent with what has been described in the literature (PMID: 26199951) 

        Exposure time: 15 seconds.

      • Immunohistochemistry (Frozen sections) - Anti-CRALBP antibody [EPR23448-119] (ab243664)
        Immunohistochemistry (Frozen sections) - Anti-CRALBP antibody [EPR23448-119] (ab243664)

        Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 peremeabilised mouse retina tissue labeling CRALBP with ab243664 at 1/250 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Positive staining on mouse retina especially on retinal pigment epithelial cell (RPE) and Müller cells. The nuclear counter stain is DAPI (Blue).

        Secondary antibody control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/000 dilution.

        Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      • Western blot - Anti-CRALBP antibody [EPR23448-119] (ab243664)
        Western blot - Anti-CRALBP antibody [EPR23448-119] (ab243664)
        Anti-CRALBP antibody [EPR23448-119] (ab243664) at 1/1000 dilution + Human eyeball tissue lysate at 20 µg

        Secondary
        VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

        Predicted band size: 36 kDa
        Observed band size: 36 kDa



        Blocking and diluting buffer and concentration: 5% NFDM/TBST.

        The molecular weight observed is consistent with what has been described in the literature (PMID: 29063836).

        Exposure time: 3.25 seconds.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] (ab243664)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] (ab243664)

        Immunohistochemical analysis of paraffin-embedded Rat retina tissue labeling CRALBP with ab243664 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat retina. The section was incubated with ab243664 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      • Immunohistochemistry (Frozen sections) - Anti-CRALBP antibody [EPR23448-119] (ab243664)
        Immunohistochemistry (Frozen sections) - Anti-CRALBP antibody [EPR23448-119] (ab243664)

        Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 peremeabilised rat retina tissue labeling CRALBP with ab243664 at 1/250 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Positive staining on rat retina especially on retinal pigment epithelial cell (RPE) and Müller cells. The nuclear counter stain is DAPI (Blue).

        Secondary antibody control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/000 dilution.

        Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] (ab243664)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] (ab243664)

        Immunohistochemical analysis of paraffin-embedded Mouse retina tissue labeling CRALBP with ab243664 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse retina. The section was incubated with ab243664 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] (ab243664)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] (ab243664)

        Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CRALBP with ab243664 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on human kidney. The section was incubated with ab243664 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab243664? Please let us know so that we can cite the reference in this datasheet.

      ab243664 has not yet been referenced specifically in any publications.

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