Anti-CRAT antibody (ab153699)

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Western blot - Anti-CRAT antibody (ab153699)
  • Immunocytochemistry/ Immunofluorescence - Anti-CRAT antibody (ab153699)

Key features and details

  • Rabbit polyclonal to CRAT
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-CRAT antibody
    See all CRAT primary antibodies

  • Description

    Rabbit polyclonal to CRAT

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 7-323 of Human CRAT (UniProt: P43155).

  • Positive control

    • 293T, H1299 and Raji whole cell lysates; H1299 cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab153699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
All applications
Mouse
Rat
Cow
Pig
Application Abreviews Notes
WB (1)
1/500 - 1/3000. Predicted molecular weight: 71 kDa.
ICC/IF (1)
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 71 kDa.
ICC/IF
1/100 - 1/1000.

Target

Images

  • Western blot - Anti-CRAT antibody (ab153699)
    Western blot - Anti-CRAT antibody (ab153699)
    Anti-CRAT antibody (ab153699) at 1/1500 dilution + Raji whole cell lysates at 30 µg

    Predicted band size: 71 kDa



    10% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-CRAT antibody (ab153699)
    Immunocytochemistry/ Immunofluorescence - Anti-CRAT antibody (ab153699)
    Immunofluorescent analysis of methanol-fixed H1299 cells labeling CRAT with ab153699 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.

References (1)

Publishing research using ab153699? Please let us know so that we can cite the reference in this datasheet.

ab153699 has been referenced in 1 publication.

  • Sanchez-Ruderisch H  et al. Sex-specific regulation of cardiac microRNAs targeting mitochondrial proteins in pressure overload. Biol Sex Differ 10:8 (2019). PubMed: 30728084

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-CRAT antibody

 Average
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Quadriceps, Kidney)
Gel Running Conditions
Reduced Denaturing (4-15%, 12%)
Loading amount
50 µg
Specification
Quadriceps, Kidney
Blocking step
Casein Fish Gelatin Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Jun 21 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-CRAT antibody

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Testis)
Permeabilization
Yes - 0,5% Triton X-100 in blocking buffer
Specification
Testis
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 37°C
Fixative
Paraformaldehyde
Read More

Miss. Marie-Chantal Grégoire

Verified customer

Submitted Jun 19 2015

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