Overview

  • Product name

  • Description

    Rabbit polyclonal to CRAT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 7-323 of Human CRAT (UniProt: P43155).

  • Positive control

    • 293T, H1299 and Raji whole cell lysates; H1299 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab153699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 71 kDa.
ICC/IF 1/100 - 1/1000.

Target

Images

  • Anti-CRAT antibody (ab153699) at 1/1500 dilution + Raji whole cell lysates at 30 µg

    Predicted band size: 71 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed H1299 cells labeling CRAT with ab153699 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.

References

This product has been referenced in:

  • Sanchez-Ruderisch H  et al. Sex-specific regulation of cardiac microRNAs targeting mitochondrial proteins in pressure overload. Biol Sex Differ 10:8 (2019). Read more (PubMed: 30728084) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Quadriceps, Kidney)
Gel Running Conditions
Reduced Denaturing (4-15%, 12%)
Loading amount
50 µg
Specification
Quadriceps, Kidney
Blocking step
Casein Fish Gelatin Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 21 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Testis)
Permeabilization
Yes - 0,5% Triton X-100 in blocking buffer
Specification
Testis
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 37°C
Fixative
Paraformaldehyde

Miss. Marie-Chantal Grégoire

Verified customer

Submitted Jun 19 2015

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