Anti-CRAT antibody (ab153699)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-CRAT antibody
See all CRAT primary antibodies -
Description
Rabbit polyclonal to CRAT -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 7-323 of Human CRAT (UniProt: P43155).
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Positive control
- 293T, H1299 and Raji whole cell lysates; H1299 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 10% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab153699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 71 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Carnitine acetylase is specific for short chain fatty acids. Carnitine acetylase seems to affect the flux through the pyruvate dehydrogenase complex. It may be involved as well in the transport of acetyl-CoA into mitochondria. -
Tissue specificity
Mostly in skeletal muscle, less in heart, liver and pancreas, only weakly detectable in brain, placenta, lung and kidney. -
Sequence similarities
Belongs to the carnitine/choline acetyltransferase family. -
Cellular localization
Mitochondrion; Peroxisome and Endoplasmic reticulum. Peroxisome. Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 512902 Cow
- Entrez Gene: 1384 Human
- Entrez Gene: 12908 Mouse
- Entrez Gene: 100127351 Pig
- Entrez Gene: 311849 Rat
- Omim: 600184 Human
- SwissProt: P43155 Human
- SwissProt: P47934 Mouse
see all -
Alternative names
- CACP_HUMAN antibody
- Carnitine acetylase antibody
- Carnitine acetyltransferase antibody
see all
Images
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Western blot - Anti-CRAT antibody (ab153699)Anti-CRAT antibody (ab153699) at 1/1500 dilution + Raji whole cell lysates at 30 µg
Predicted band size: 71 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-CRAT antibody (ab153699)Immunofluorescent analysis of methanol-fixed H1299 cells labeling CRAT with ab153699 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
References
This product has been referenced in:
- Sanchez-Ruderisch H et al. Sex-specific regulation of cardiac microRNAs targeting mitochondrial proteins in pressure overload. Biol Sex Differ 10:8 (2019). Read more (PubMed: 30728084) »
