Overview

  • Product name

  • Description

    Rabbit polyclonal to CRAT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 388-609 of Human CRAT.

  • Positive control

    • 293T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab153750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 71 kDa.

Target

Images

  • Anti-CRAT antibody (ab153750) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 71 kDa



    7.5% SDS PAGE
  • All lanes : Anti-CRAT antibody (ab153750) at 1/1000 dilution

    Lane 1 : Wildtype C57BL/6J quadriceps muscle whole cell lysate
    Lane 2 : CrAT Knockout C57BL/6J quadriceps muscle whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Alexa Fluor® 680-conjugated goat anti-rabbit IgG (H+L) polyclonal at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 71 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 150 kDa (possible non-specific binding), 37 kDa (possible non-specific binding)


    Exposure time: 1 minute


    The CrAT knockout was achieved in C57BL6/J transgenic animals expressing a tamoxifen-inducible Cre whose CrAT alleles were homozygously floxed. This model was previously validated through genome sequencing, mRNA expression, and protein expression

    • Western Membrane: Nitrocellulose 0.45µm
    • Lysis buffer: 20mM Tris-HCl, 4% SDS, 10mM NaF, 1mM EDTA, 20% glycerol

    See Abreview

References

ab153750 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-15% in T/G/SDS-buffer)
Sample
Mouse Cell lysate - whole cell (Skeletal Muscle)
Specification
Skeletal Muscle
Blocking step
Casein Fish Gelatin Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 03 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 37°C
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K (4´g/mL, 15 min, 37¯C)
Sample
Mouse Tissue sections (Paraffin-embedded testis section)
Specification
Paraffin-embedded testis section
Permeabilization
Yes - 0,1% Triton X-100 in blocking buffer
Fixative
Formaldehyde

Miss. Marie-Chantal Grégoire

Verified customer

Submitted Mar 17 2014

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