Overview

  • Product name

    Anti-CRBN antibody - N-terminal
    See all CRBN primary antibodies
  • Description

    Rabbit polyclonal to CRBN - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human CRBN (N terminal). The exact sequence is proprietary.

  • Positive control

    • ICC/IF: SK-N-SH cells. WB: U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 51 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Component of some DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8. In the complex, may act as a substrate receptor. Regulates the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1.
  • Tissue specificity

    Widely expressed. Highly expressed in brain.
  • Pathway

    Protein modification; protein ubiquitination.
  • Involvement in disease

    Defects in CRBN are the cause of mental retardation autosomal recessive type 2A (MRT2A) [MIM:607417]. MRT2A patients display mild mental retardation with a standard IQ ranged from 50 to 70. IQ scores are lower in males than females. Developmental milestones are mildly delayed. There are no dysmorphic or autistic features. Non-syndromic mental retardation patients do not manifest other clinical signs.
  • Sequence similarities

    Belongs to the CRBN family.
    Contains 1 Lon domain.
  • Post-translational
    modifications

    Ubiquitinated, ubiquitination is mediated by its own DCX protein ligase complex.
  • Cellular localization

    Cytoplasm. Nucleus. Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2610203G15Rik antibody
    • 2900045O07Rik antibody
    • AF229032 antibody
    • AW108261 antibody
    • Cereblon antibody
    • Crbn antibody
    • CRBN_HUMAN antibody
    • DKFZp781K0715 antibody
    • MGC27358 antibody
    • MRT2A antibody
    • OTTHUMP00000209555 antibody
    • piL antibody
    • Protein cereblon antibody
    • Protein x 0001 antibody
    see all

Images

  • 4% paraformaldehyde-fixed SK-N-SH (human neuroblastoma cell line) cells stained for CRBN (green) using ab226782 at 1/500 dilution in ICC/IF. Blue: Hoechst 33342 staining.

  • All lanes : Anti-CRBN antibody - N-terminal (ab226782) at 1/1000 dilution

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
    Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
    Lane 3 : IMR32 whole cell extract
    Lane 4 : SK-N-AS whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 51 kDa



    10% SDS-PAGE gel.

References

ab226782 has not yet been referenced specifically in any publications.

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