Key features and details
- Rabbit polyclonal to CRBN - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CRBN antibody - N-terminal
See all CRBN primary antibodies
DescriptionRabbit polyclonal to CRBN - N-terminal
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Rhesus monkey
Recombinant fragment within Human CRBN (N terminal). The exact sequence is proprietary.
- ICC/IF: SK-N-SH cells. WB: U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226782 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 51 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionComponent of some DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8. In the complex, may act as a substrate receptor. Regulates the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1.
Tissue specificityWidely expressed. Highly expressed in brain.
PathwayProtein modification; protein ubiquitination.
Involvement in diseaseDefects in CRBN are the cause of mental retardation autosomal recessive type 2A (MRT2A) [MIM:607417]. MRT2A patients display mild mental retardation with a standard IQ ranged from 50 to 70. IQ scores are lower in males than females. Developmental milestones are mildly delayed. There are no dysmorphic or autistic features. Non-syndromic mental retardation patients do not manifest other clinical signs.
Sequence similaritiesBelongs to the CRBN family.
Contains 1 Lon domain.
modificationsUbiquitinated, ubiquitination is mediated by its own DCX protein ligase complex.
Cellular localizationCytoplasm. Nucleus. Membrane.
- Information by UniProt
- 2610203G15Rik antibody
- 2900045O07Rik antibody
- AF229032 antibody
4% paraformaldehyde-fixed SK-N-SH (human neuroblastoma cell line) cells stained for CRBN (green) using ab226782 at 1/500 dilution in ICC/IF. Blue: Hoechst 33342 staining.
All lanes : Anti-CRBN antibody - N-terminal (ab226782) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 3 : IMR32 whole cell extract
Lane 4 : SK-N-AS whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 51 kDa
10% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226782 has not yet been referenced specifically in any publications.