Overview

  • Product name
    Anti-Cre recombinase antibody [7.23]
    See all Cre recombinase primary antibodies
  • Description
    Mouse monoclonal [7.23] to Cre recombinase
  • Host species
    Mouse
  • Specificity
    Current Batch not validated for Immunohistochemistry.
  • Tested applications
    Suitable for: WBmore details
  • Immunogen

    Full length native protein (affinity purified).

  • General notes


    This antibody can be used to determine the success of gene manipulations in transgenic animals.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.03% Thimerosal (merthiolate)
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity
    Protein G purified
  • Primary antibody notes
    This antibody can be used to determine the success of gene manipulations in transgenic animals.
  • Clonality
    Monoclonal
  • Clone number
    7.23
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa).

Target

  • Relevance
    Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family.
  • Alternative names
    • Cre antibody
    • Cyclization recombinase antibody
    • Recombinase cre antibody

Images

  • All lanes : Anti-Cre recombinase antibody [7.23] (ab24607) at 1/1000 dilution

    Lane 1 : Escherichia coli expressing Cre-recombinase
    Lane 2 : Hek cell expressing control plasmid

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-mouse HRP conjugated at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 38 kDa
    Observed band size: 34 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 50&80 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute

References

This product has been referenced in:
  • Chen E  et al. Altered Baseline and Nicotine-Mediated Behavioral and Cholinergic Profiles in ChAT-Cre Mouse Lines. J Neurosci 38:2177-2188 (2018). Read more (PubMed: 29371319) »
  • Wu Y  et al. Ga12 is required for renal cystogenesis induced by Pkd1 inactivation. J Cell Sci 129:3675-3684 (2016). Read more (PubMed: 27505895) »
See all 15 Publications for this product

Customer reviews and Q&As

1-10 of 18 Abreviews or Q&A

Answer

Thank you for contacting us.

Your credit note ID is ***.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (Brain tissue (Inferior colliculus and Medial Genic)
Specification
Brain tissue (Inferior colliculus and Medial Genic

Abcam user community

Verified customer

Submitted Jun 14 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mouse embryonic spinal cord (cryosections))
Permeabilization
Yes - 0.1%Triton X-100
Specification
mouse embryonic spinal cord (cryosections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde

Dr. Frederic Clotman

Verified customer

Submitted Nov 19 2015

Answer

Thank you for contacting us.

Please check the following;

http://www.ncbi.nlm.nih.gov/pubmed/18408763?dopt=Abstract

It is also used in other publications but we are unable to confirm the applications due to non availability of access to these articles.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Ante todo quisiera desearle un muy Feliz Año.

Muchas gracias por tomarte la molestia de rellenar el cuestionario y mandar más imágenes.

Hay alguna sugerencia que posiblemente mejore la tinción obtenida. Me decías en el primer correo que se había probado ya la recuperación antigénica del tejido tras fijación con 4%PFA durante la noche. Es posible que fijando las secciones congeladas con acetona a temperatura ambiente durante 10 minutos se observe mayor tinción. Además, la acetona tiene efecto permeabilizador en las células, facilitando la inclusión del anticuerpo en las mismas.

Otra recomendación que quisiera hacer es probar de bloquear el tejido de forma más suave. Se está utilizando un porcentaje muy alto de suero (16%), y los anticuerpos además se diluyen en el mismo.

Si podéis usar una dilución menos concentrada (10%) y diluir los anticuerpos en TBST con un porcentaje bajo (1%) de BSA, quizás la membrana no quede tan bloqueada y se permita una mejor visualización de la tinción.

En cualquier caso, si preferís que apliquemos directamente la garantía, puesto que ya habíais realizado pasos de optimización, y que os mandemos un reemplazo del anticuerpo (del mismo u otro diferente) o reembolso del importe del mismo, no dudes en indicármelo y estaré encantada de poder ayudar.

Espero entonces tu respuesta para proceder y quedo a la disposición de cualquier otra consulta que podáis tener.

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Answer

Thank you for contacting us.

Perhaps these are the antibodies customer is looking for;

https://www.abcam.com/cre-recombinase-antibody-723-ab24607.html

https://www.abcam.com/cre-recombinase-antibody-723-biotin-ab24580.html

https://www.abcam.com/cre-recombinase-antibody-ab40011.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Ich möchte Ihnen hiermit nochmals viel Erfolg wünschen und drücke die Daumen, dass dieser Antikörper funktioniert.

Wenn Sie Ihre Versuche abgeschlossen haben würden wir uns über einen Abreview freuen. Die gesammelten Abpoints können Sie für weitere Abcamprodukte einlösen oder auch in Amazongutscheine eintauschen. Wenn Sie mehr über unser Abreviewsystem erfahren wollen schauen Sie bitte https://www.abcam.com/index.html?pageconfig=resource&rid=10332&viapagetrap=abreviews.

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Answer

Vielen Dank für diese zusätzliche Information.

Leider haben wir keinen Antikörper den ich für die Detektion Ihres rekombinanten Proteins garantieren kann. Ich habe drei Antikörper gefunden die vielleicht für Sie in Frage kommen und die Sie sich genauer anschauen sollten.

1. https://www.abcam.com/cre-recombinase-antibody-723-ab24607.html

Immunogensequenz ist das gesamte native Cre Rekombinase Protein. Die Sequenz sollte daher mit der Sequenz in dem rekombinanten Protein übereinstimmen.

2. https://www.abcam.com/cre-recombinase-antibody-ab137240.html

Bei dem Immunogen handelt es sich um ein rekombinantes full length protein des Bacteriophage P1 Cre recombinase.

3. https://www.abcam.com/progesterone-receptor-antibody-alpha-pr6-ab2765.html

Die Immunogensequenz dieses Antikörpers ist leider nicht näher bestimmt. Der Antikörper wurde gegen das gesamte Hühnchen Progesteron gerichtet hergestellt. Die Hühnchensequenz verglichen mit der zur Verfügung gestellten Progesteronsequenz des rekombinanten Proteins weißt eine Uebereinstimmung von 78% auf. Die Detektion eines Proteins werten wir als wahrscheinlich, wenn eine Übereinstimmung von etwa 85% besteht.



Der ab24607 ist unter diesen drei Möglichkeiten der mit den meisten Referenzen und eingereichten Abreviews und daher am besten charakterisiert. Ab24607 wurde schon für IHC-Fr in Maus verwendet und zwei Abreviews speziell hierfür wurden verfasst.

Ich hoffe ich konnte Ihnen etwas weiter helfen. Es tut mir Leid dass ich Ihnen in diesem Fall kein perfektes Produkt anbieten kann.

Sollten Sie sich dafür entscheiden einen dieser Antikörper zu kaufen, möchte ich Sie noch auf unsere RabMab Aktion aufmerksam machen. Bitte lesen Sie mehr über diese Aktionhttps://www.abcam.com/index.html?pageconfig=resource&rid=15447.

Bitte zögern Sie nicht sich wieder bei mir zu melden, sollten Sie weitere Fragen oder Probleme mit unseren Produkten haben.

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Answer

Thank you for contacting us.

We have 2 antibodies in our catalogwhich have beentested in IHC-Fr: ab24607, ab24580 (biotinylated).

https://www.abcam.com/cre-recombinase-antibody-723-biotin-ab24580.html
https://www.abcam.com/cre-recombinase-antibody-723-ab24607.html

As long as cre-recombinase is expressed in the cells or tissue - independent of the species of the samples - the anti-cre antibodies will work and are guaranteed in the applications listed as tested on the datasheet.

We guarantee all our products for TESTED species and applications as listed on the datasheet. The guarantee is valid for 6 months after a purchase. For more details about our Abpromise guarantee please check this link: https://www.abcam.com/abpromise.


If you like to try any of the other cre-antibodies that have not yet tested in IHC-Fr (ab40011, ab10171, ab41104), in that case you would be eligible for our testing discount program:

For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the antibody/protein/kit at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody/protein at the full price (100%) of the antibody/protein you have tested, or a full price discount for the amount paid for the kit. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
This programapplies to ELISA kits and other kits we offer.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question
Answer

Thank you for your reply.


I am happy to offer a replacement from another lot or a credit for this antibody. Please let me know which you would prefer.

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1-10 of 18 Abreviews or Q&A

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