Overview

  • Product name

    Anti-Cre recombinase antibody
    See all Cre recombinase primary antibodies
  • Description

    Goat polyclonal to Cre recombinase
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Immunogen

    Recombinant fragment corresponding to Cre recombinase. Enterobacteria phage P1 (Bacteriophage P1); produced in E. coli.
    Database link: P06956

  • Positive control

    • 293HEK cells transduced with Cre recombinase.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 20% Glycerol, 79% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 39 kDa.

Target

  • Relevance

    Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family.
  • Alternative names

    • Cre antibody
    • Cyclization recombinase antibody
    • Recombinase cre antibody

Images

  • Anti-Cre recombinase antibody (ab192441) at 1/2000 dilution + 293HEK cell lysate, transduced with Cre recombinase-His tag

    Secondary
    rabbit anti-goat HRP at 1/10000 dilution

    Predicted band size: 39 kDa

References

ab192441 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis from UBC-CreERT2)
Permeabilization
Yes - 0.04 % Tween20
Specification
Testis from UBC-CreERT2
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 16 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK cells)
Permeabilization
Yes - Methanol
Specification
HEK cells
Blocking step
2% BSA, 5% Donkey serum in PBS-T as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 02 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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