Product nameAnti-Cre recombinase antibody
See all Cre recombinase primary antibodies
DescriptionRabbit polyclonal to Cre recombinase
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Recombinant fragment corresponding to Bacteriophage P1 Cre recombinase aa 1-180. The immunogen was tagged with a 6xHis tag and an S tag.
MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLSVCR SWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHR RSGLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLME NSDRCQDIRNLAFLGIAYNTLLRIAEIARI
Database link: P06956
- 293T transfected with GFP-Cre recombinase; Human 293T cells transfected with GFP-Cre recombinase lysate;
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Our Abpromise guarantee covers the use of ab216262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 39 kDa.|
|ICC/IF||1/1000 - 1/2000.
Use antigen retrieval with microwave boiling for 7 minutes in 10mM Tris HCl, pH 9.
RelevanceCre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family.
- Cre antibody
- Cyclization recombinase antibody
- Recombinase cre antibody
All lanes : Anti-Cre recombinase antibody (ab216262) at 1/2000 dilution
Lane 1 : Human 293T cell lysate
Lane 2 : Human 293T cells transfected with GFP-Cre recombinase lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 39 kDa
Immunocytochemistry analysis of formalin fixed and paraffin-embedded 239T transfected with empty vector (left) or with GFP-Cre recombinase (right) labeling Cre recombinase using ab216262 at 1/1000 dilution, after antigen retrieval.
Immunofluorescent analysis of formalin fixed 293T cells transfected with GFP-Cre recombinase (green) and incubated with ab216262 at 1/2000 dilution (red). DAPI staining is shown in blue.
Flow cytometric analysis of formalin fixed 293T non transfected (top) or transfected with GFP-Cre recombinase (bottom) labeling Cre recombinase using ab216262 at 1/200 dilution, followed by Alexa 647 conjugated secondary antibody detection.
ab216262 has not yet been referenced specifically in any publications.