Anti-Cre recombinase antibody [DF6] (ab101071)
Key features and details
- Mouse monoclonal [DF6] to Cre recombinase
- Suitable for: WB
- Reacts with: Bacteriophage P1
- Isotype: IgM
Overview
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Product name
Anti-Cre recombinase antibody [DF6]
See all Cre recombinase primary antibodies -
Description
Mouse monoclonal [DF6] to Cre recombinase -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Bacteriophage P1 -
Immunogen
Synthetic peptide corresponding to Cre recombinase.
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Positive control
- This antibody gave a positive signal against a Cre Recombinase recombinant protein.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: None
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Concentration information loading...
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Purity
Concentrated Culture Supernatant -
Clonality
Monoclonal -
Clone number
DF6 -
Isotype
IgM -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab101071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). |
Target
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Relevance
Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family. -
Database links
- Entrez Gene: 2777477 Bacteriophage P1
- SwissProt: P06956 Bacteriophage P1
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Alternative names
- Cre antibody
- Cyclization recombinase antibody
- Recombinase cre antibody
Images
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All lanes : Anti-Cre recombinase antibody [DF6] (ab101071) at 1 µg/ml
Lane 1 : Cre Recombinase recombinant protein at 1 µg
Lane 2 : Cre Recombinase recombinant protein at 2 µg
Secondary
All lanes : Peroxidase- conjugated AffiniPure Goat Anti-mouse IgM at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 20 minutes
Datasheets and documents
References (0)
ab101071 has not yet been referenced specifically in any publications.