Anti-Cre recombinase antibody [DF6] (ab101071)

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Western blot - Anti-Cre recombinase antibody [DF6] (ab101071)

    Key features and details

    • Mouse monoclonal [DF6] to Cre recombinase
    • Suitable for: WB
    • Reacts with: Bacteriophage P1
    • Isotype: IgM

    Overview

    • Product name

      Anti-Cre recombinase antibody [DF6]
      See all Cre recombinase primary antibodies

    • Description

      Mouse monoclonal [DF6] to Cre recombinase

    • Host species

      Mouse

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Bacteriophage P1

    • Immunogen

      Synthetic peptide corresponding to Cre recombinase.

    • Positive control

      • This antibody gave a positive signal against a Cre Recombinase recombinant protein.

    • General notes

      This antibody clone is manufactured by Abcam.

      If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • Storage buffer

      Preservative: None
    • Concentration information loading...

    • Purity

      Concentrated Culture Supernatant

    • Clonality

      Monoclonal

    • Clone number

      DF6

    • Isotype

      IgM

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab101071 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).

    Target

    • Relevance

      Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family.

    • Database links

      • Alternative names

        • Cre antibody
        • Cyclization recombinase antibody
        • Recombinase cre antibody

      Images

      • Western blot - Anti-Cre recombinase antibody [DF6] (ab101071)
        Western blot - Anti-Cre recombinase antibody [DF6] (ab101071)
        All lanes : Anti-Cre recombinase antibody [DF6] (ab101071) at 1 µg/ml

        Lane 1 : Cre Recombinase recombinant protein at 1 µg
        Lane 2 : Cre Recombinase recombinant protein at 2 µg

        Secondary
        All lanes : Peroxidase- conjugated AffiniPure Goat Anti-mouse IgM at 1/5000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 38 kDa
        Observed band size: 38 kDa


        Exposure time: 20 minutes

      References (0)

      Publishing research using ab101071? Please let us know so that we can cite the reference in this datasheet.

      ab101071 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      1-3 of 3 Abreviews or Q&A

      Question

      No bands in Western blot with transgenic mouse samples.

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      Answer

      Thank you for your call today and for letting us know about the trouble with this antibody.

      As we discussed, please let me know about the results after using the anti-mouse IgM secondary antibody. If there are still no bands on the blot, I will be happy to help you further.

      I look forward to hearing from you. If you have any questions or if there is anything else that we can do for you, please let me know.

      Have a nice day.

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      Question

      In my lab we are interested in purchasing a good Cre-recombinase antibody. For years we used another antibody but this product is not available anymore. Therefore, we would first like to obtain small aliquots of your anti-Cre recombinase antibodies ab40011,ab41104 and ab101071 for testing in our immunohistochemistry and immunofluorescence protocols for mouse brains. In case you had an antibody derived from full-length recombinant Cre, we would be very interested. We can make the testing data available to you. And if an antibody works well for our purposes, we would like to order a large batch of it. I look forward to a positive offer and wish you a nice day.

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      Answer

      Thank you for contacting us.

      Unfortunately, because we carry over 80,000 products, it isn't feasible for us to keep small sample sizes of our products.

      However, we are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase. This means for the mentioned anti-Cre recombinase antibodies that we guarantee them to be specific for cre-recombinase and to detect the protein in Western blot.

      To our knowledge, these antibodies have not been tested in immunohistochemistry (IHC) and immunofluorescence (IF) yet, but there is no reason why they should not work. Therefore, I can offer a discount off a future purchase if you buy one of these antibodies now, test it in IHC and/or IF and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.


      If you are interested in this offer, please follow these steps:

      1. Reply to this e-mail to let me know if you would like to proceed and which antibody you would like to test in IHC/IF. I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering.

      2. Purchase the antibody either by phone, fax, or online (www.abcam.com).

      3. Test it in IHC/IF.

      4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews.

      5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

      We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the antibody turns out to be unsuitable for IHC/IF, you will still receive the discount on your next purchase after your Abreview has been submitted.

      Once you have found a suitable antibody for your experimental settings, we would be pleased to make you an offer for a larger order. So please do not hesitate to contact us with further details.

      Please let me know if you have any questions about this offer and I would be happy to help you further.

      The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount

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      Question

      I would like to test this antibody in immunohistochemistry PFA perfusion fixed frozen sections (IHC-FoFr), an untested application.

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      Answer

      I am very pleased to hear you would like to accept our offer and test ab101071 in IHC-FoFr.   This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IHC-FoFr and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

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      For licensing inquiries, please contact partnerships@abcam.com