Recombinant Anti-Cre recombinase antibody [EPR13835] (ab188568)
![Western blot - Anti-Cre recombinase antibody [EPR13835] (ab188568)](https://www.abcam.com/ps/products/188/ab188568/Images/ab188568-222526-anti-cre-recombinase-antibody-epr13835-western-blot.jpg "Western blot - Anti-Cre recombinase antibody [EPR13835] (ab188568)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13835] to Cre recombinase
- Suitable for: WB
- Reacts with: Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-Cre recombinase antibody [EPR13835]
See all Cre recombinase primary antibodies -
Description
Rabbit monoclonal [EPR13835] to Cre recombinase -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13835 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 39 kDa. |
Target
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Relevance
Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family. -
Alternative names
- Cre antibody
- Cyclization recombinase antibody
- Recombinase cre antibody
Images
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Western blot - Anti-Cre recombinase antibody [EPR13835] (ab188568)All lanes : Anti-Cre recombinase antibody [EPR13835] (ab188568) at 1/5000 dilution
Lane 1 :Recombinant Bacteriophage P1 Cre recombinase protein (ab134845) at 0.001 µg
Lane 2 : E. coli at 10 µg
Lane 3 : Saccharomyces cerevisiae at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 39 kDa -
![Anti-Cre recombinase antibody [EPR13835] (ab188568)](https://www.abcam.com/ps/products/188/ab188568/Images/ab188568-2-benefits-of-recombinant-antibodies.png)
Anti-Cre recombinase antibody [EPR13835] (ab188568)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab188568 has been referenced in 2 publications.
- He L et al. A protocol for in vivo analysis of liver tumorigenesis in mice using sleeping beauty transposon system. STAR Protoc 2:100445 (2021). PubMed: 33912846
- Chen E et al. Altered Baseline and Nicotine-Mediated Behavioral and Cholinergic Profiles in ChAT-Cre Mouse Lines. J Neurosci 38:2177-2188 (2018). PubMed: 29371319