Recombinant
RabMAb

Recombinant Anti-Cre recombinase antibody [EPR13835] (ab188568)

Rabbit recombinant monoclonal Cre recombinase antibody [EPR13835]. Validated in WB. Cited in 1 publication(s).

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Overview

  • Product name

    Anti-Cre recombinase antibody [EPR13835]
    See all Cre recombinase primary antibodies

  • Description

    Rabbit monoclonal [EPR13835] to Cre recombinase

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Immunogen

    Synthetic peptide within Cre recombinase aa 1-100. The exact sequence is proprietary.
    Database link: P06956

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13835

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab188568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.

Target

  • Relevance

    Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family.

  • Alternative names

    • Cre antibody
    • Cyclization recombinase antibody
    • Recombinase cre antibody

Images

  • Western blot - Anti-Cre recombinase antibody [EPR13835] (ab188568)
    Western blot - Anti-Cre recombinase antibody [EPR13835] (ab188568)
    All lanes : Anti-Cre recombinase antibody [EPR13835] (ab188568) at 1/5000 dilution

    Lane 1 : Recombinant Bacteriophage P1 Cre recombinase protein (ab134845) at 0.001 µg
    Lane 2 : E. coli at 10 µg
    Lane 3 : Saccharomyces cerevisiae at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 39 kDa

References

This product has been referenced in:

  • Chen E  et al. Altered Baseline and Nicotine-Mediated Behavioral and Cholinergic Profiles in ChAT-Cre Mouse Lines. J Neurosci 38:2177-2188 (2018). Read more (PubMed: 29371319) »
See 1 Publication for this product

Publishing research using ab188568? Please let us know so that we can cite the reference in this datasheet.

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