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  1. Link

    cre-recombinase-antibody-epr13835-ab188568.pdf

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Tags & Cell Markers Fusion / Marker Proteins Cre / Lox
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RecombinantRabMAb

Recombinant Anti-Cre recombinase antibody [EPR13835] (ab188568)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question References (2)

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Western blot - Anti-Cre recombinase antibody [EPR13835] (ab188568)
  • Anti-Cre recombinase antibody [EPR13835] (ab188568)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13835] to Cre recombinase
  • Suitable for: WB
  • Reacts with: Recombinant fragment

Conjugates logo Related conjugates and formulations

Carrier Free

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Recombinant Bacteriophage P1 Cre recombinase protein (ab134845)
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Overview

  • Product name

    Anti-Cre recombinase antibody [EPR13835]
    See all Cre recombinase primary antibodies
  • Description

    Rabbit monoclonal [EPR13835] to Cre recombinase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Recombinant fragment
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13835
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Fusion / Marker Proteins
    • Cre / Lox

Associated products

  • Alternative Versions

    • Anti-Cre recombinase antibody [EPR13835] - BSA and Azide free (ab240304)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Bacteriophage P1 Cre recombinase protein (ab134845)
  • Related Products

    • Recombinant Bacteriophage P1 Cre recombinase protein (ab134845)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab188568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 39 kDa.

Target

  • Relevance

    Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family.
  • Alternative names

    • Cre antibody
    • Cyclization recombinase antibody
    • Recombinase cre antibody

Images

  • Western blot - Anti-Cre recombinase antibody [EPR13835] (ab188568)
    Western blot - Anti-Cre recombinase antibody [EPR13835] (ab188568)
    All lanes : Anti-Cre recombinase antibody [EPR13835] (ab188568) at 1/5000 dilution

    Lane 1 : Recombinant Bacteriophage P1 Cre recombinase protein (ab134845) at 0.001 µg
    Lane 2 : E. coli at 10 µg
    Lane 3 : Saccharomyces cerevisiae at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 39 kDa

  • Anti-Cre recombinase antibody [EPR13835] (ab188568)
    Anti-Cre recombinase antibody [EPR13835] (ab188568)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (2)

Publishing research using ab188568? Please let us know so that we can cite the reference in this datasheet.

ab188568 has been referenced in 2 publications.

  • He L  et al. A protocol for in vivo analysis of liver tumorigenesis in mice using sleeping beauty transposon system. STAR Protoc 2:100445 (2021). PubMed: 33912846
  • Chen E  et al. Altered Baseline and Nicotine-Mediated Behavioral and Cholinergic Profiles in ChAT-Cre Mouse Lines. J Neurosci 38:2177-2188 (2018). PubMed: 29371319

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