RecombinantRabMAb

Recombinant Anti-Cre recombinase antibody [EPR13835] (ab188568)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13835] to Cre recombinase
  • Suitable for: WB
  • Reacts with: Recombinant fragment

Overview

  • Product name

    Anti-Cre recombinase antibody [EPR13835]
    See all Cre recombinase primary antibodies

  • Description

    Rabbit monoclonal [EPR13835] to Cre recombinase

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Recombinant fragment

  • Immunogen

    Synthetic peptide within Cre recombinase aa 1-100. The exact sequence is proprietary.
    Database link: P06956

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13835

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab188568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.

Target

  • Relevance

    Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family.

  • Alternative names

    • Cre antibody
    • Cyclization recombinase antibody
    • Recombinase cre antibody

Images

  • Western blot - Anti-Cre recombinase antibody [EPR13835] (ab188568)
    Western blot - Anti-Cre recombinase antibody [EPR13835] (ab188568)
    All lanes : Anti-Cre recombinase antibody [EPR13835] (ab188568) at 1/5000 dilution

    Lane 1 : Recombinant Bacteriophage P1 Cre recombinase protein (ab134845) at 0.001 µg
    Lane 2 : E. coli at 10 µg
    Lane 3 : Saccharomyces cerevisiae at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 39 kDa

  • Anti-Cre recombinase antibody [EPR13835] (ab188568)
    Anti-Cre recombinase antibody [EPR13835] (ab188568)

References (1)

Publishing research using ab188568? Please let us know so that we can cite the reference in this datasheet.

ab188568 has been referenced in 1 publication.

  • Chen E  et al. Altered Baseline and Nicotine-Mediated Behavioral and Cholinergic Profiles in ChAT-Cre Mouse Lines. J Neurosci 38:2177-2188 (2018). PubMed: 29371319

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