Recombinant Anti-Cre recombinase antibody [EPR13835] - BSA and Azide free (ab240304)
![Western blot - Anti-Cre recombinase antibody [EPR13835] - BSA and Azide free (ab240304)](https://www.abcam.com/ps/products/240/ab240304/Images/ab240304-398988-anti-cre-recombinase-antibody-epr13835-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-Cre recombinase antibody [EPR13835] - BSA and Azide free (ab240304)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13835] to Cre recombinase - BSA and Azide free
- Suitable for: WB
- Reacts with: Recombinant fragment
Overview
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Product name
Anti-Cre recombinase antibody [EPR13835] - BSA and Azide free
See all Cre recombinase primary antibodies -
Description
Rabbit monoclonal [EPR13835] to Cre recombinase - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab240304 is the carrier-free version of ab188568. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab240304 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13835 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab240304 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 39 kDa.
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| Notes |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 39 kDa. |
Target
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Relevance
Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family. -
Alternative names
- Cre antibody
- Cyclization recombinase antibody
- Recombinase cre antibody
Images
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Western blot - Anti-Cre recombinase antibody [EPR13835] - BSA and Azide free (ab240304)All lanes : Anti-Cre recombinase antibody [EPR13835] (ab188568) at 1/5000 dilution
Lane 1 : Active bacteriophage p1 Cre recombinase full length protein at 0.001 µg
Lane 2 : E. coli at 10 µg
Lane 3 : Saccharomyces cerevisiae at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 39 kDaThis data was developed using ab188568, the same antibody clone in a different buffer formulation.
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![Anti-Cre recombinase antibody [EPR13835] - BSA and Azide free (ab240304)](https://www.abcam.com/ps/products/240/ab240304/Images/ab240304-2-benefits-of-recombinant-antibodies.png)
Anti-Cre recombinase antibody [EPR13835] - BSA and Azide free (ab240304)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab240304 has not yet been referenced specifically in any publications.
