Question (37855) | Creatine Assay Kit (Colorimetric/Fluorometric) (ab65339)

Go to datasheet (ab65339)

Question

We are trying find whatare difference between Cat# ab65339 and ab6540.

I am looking forward tyo hearing from you.

Answer

Thank you for your inquiry.


The ab65339 kit measures creatine, while the ab65340 kit measures creatinine.


https://www.abcam.com/Creatine-Assay-Kit-ab65339.html


https://www.abcam.com/Creatinine-Assay-Kit-ab65340.html


The Creatine Assay Kit provides an accurate, convenient measure of creatine in a variety of biological samples. In the assay, creatine is enzymatically converted to sarcosine which is then specifically oxidized to generate a product that converts a colorless probe to an intensely red color (detected by spectrophotometry at OD = 570 nm), and highly fluorescent (Ex/Em = 538/587 nm) product. Creatine is therefore easily detected by either colorimetric or fluorometric methods. Detection range 0.001 – 10 mM Creatine.


The Creatinine Assay Kit provides an accurate, convenient measure of creatinine concentration in biological fluids such as serum, urine or CSF. In the assay, creatinine is converted to creatine by creatininase, creatine is converted to sarcosine, which is specifically oxidized to produce a product which reacts with a probe to generate red color (detected by spectrophotometry at OD = 570 nm) and fluorescence (Ex/Em = 538/587 nm). This kit is suitable for serum/plasma creatinine determinations, as well as for urine and other biological samples.


Essentially the only difference is that the creatinine kit involves an additional step involving creatininase to create creatine.


I hope this information helps. Please contact us with any other questions.

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