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Product code: 65339
Inquiry: I need to measure the levels of phosphocreatine in a cell homogenates of mouse heart. Do you think I could use the kit for measuring creatine? Sincerely
Asked on Jun 14 2012
Thank you for contacting us.
I have to confirm that we have not specifically tested with this sample type (tissue) and it is therefore not covered by our guarantee.
This kit should be compatible with all mammalian samples and therefore should also theoretically work with mouse heart cell lysates.
I suggest to homogenize the tissue in assay buffer with a dounce homogenizer. This will have to be optimized by the end user.
High concentrations of proteins in lysates may interfere with the assay. Samples containing proteins may be filtered through a 10k MW cut-off filter:
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Jun 14 2012