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Is this kit suitable for differentiation between phospho and non phospho form of creatine?
Asked on Oct 04 2012
Thank you for your phone call.
I can confirm that this kit will not be able to distinguish between phospho-creatine and creatine molecules. The enzyme used in this kit Creatine Amidinohydrolase which converts creatine into Sarcosine.
If you are interested in determining the level of phpspho-creatine then one way is converting all the phospho-creatine to creatine using Creatinine Amidohydrolase enzyme.
By subtracting the value of creatine from total creatine, will give you the phospho-creatine level.
I hope this information will be helpful. Should you have any other question please do not hesitate to contact me.
Answered on Oct 04 2012