Question (75553) | Creatine Assay Kit (Colorimetric/Fluorometric) (ab65339)

Go to datasheet (ab65339)

Question

The kit detect only non-phoshorylated creatine, correct? I saw the information that says total creatine can be detected using Creatinine Amidohydrolase enzyme. Could you tell me the detail protocol for that?

Answer

Sorry for any confusion here.
In the assay, creatine is enzymatically converted to sarcosine which is catalyzed by creatinase. This enzyme works at the first step of the detection reaction in the kit. This conversion event is not dependent on phosphorylation of creatine. So in summary this kit can't be used to distinguish between phosphocreatine unphosphorylated creatine.

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