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Creatine Kinase Activity Assay kit (Colorimetric) (ab155901) is a kit where creatine kinase (CK) converts creatine into phosphocreatine and ADP. The generated phoshocreatine and ADP reacts with CK Enzyme Mix to form an intermediate, which reduces a colorless probe to a colored product with strong absorbance at ?= 450 nm. The CK Activity Assay is high-throughput adaptable, simple and sensitive. This assay kit can detect CK activity less than 1 mU.
Creatine Kinase (CK) also known as creatine phosphokinase (CPK) and ATP: creatine N-phosphotransferase is a common cellular enzyme (EC 184.108.40.206). It catalyzes the reversible conversion of creatine and ATP into ADP and phosphocreatine. CK is widely expressed in various tissues and cell types, with highest activity in striated muscles, heart tissue and brain. CK consists of two subunits: M (muscle) and B (brain), and has three isoenzymes: CK-MM (skeleton muscle), CK-MB (cardiac muscle), and CK-BB (brain). Increased CK level is associated with many diseases such as myocardial infarction, muscular dystrophy, pulmonary infarction and brain tumors. Accurate measurement of CK is crucial for early diagnosis, prediction and therapeutic strategy.
|CK Assay Buffer||WM||1 x 25ml|
|CK Developer||Red||1 vial|
|CK Enzyme Mix||Green||1 vial|
|CK Positive Control||Purple||1 vial|
|CK Substrate||Blue||1 x 1ml|
|NADH Standard||Yellow||1 vial|
Creatine Kinase Activity measured in various samples.
Initial protein concentration for tissue lysates varied from 1 mg/mL to 3 mg/mL. 40 μl of filtered tissue lysates were used. 30 μl of filtered biological fluids were used.
Typical NADH standard calibration curve using colorimetric reading.
Creatine kinase activity tested in 5 µl human serum and 192 ng rat heart lysate. Assay performed following kit protocol.
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