Creatine Kinase Activity Assay Kit (Colorimetric) (ab155901)
- Datasheet
- Specific References (5)
- Protocols
Overview
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Product nameCreatine Kinase Activity Assay Kit (Colorimetric)
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Sample typeSerum, Plasma, Tissue, Adherent cells, Suspension cells, Tissue Homogenate
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Assay typeEnzyme activity
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Sensitivity< 1 mU/well
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Range2 nmol/well - 10 nmol/well
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Assay time1h 0m
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Species reactivityReacts with: Mammals, Other species
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Product overview
Creatine Kinase Activity Assay kit (Colorimetric) (ab155901) is a kit where creatine kinase (CK) converts creatine into phosphocreatine and ADP. The generated phoshocreatine and ADP reacts with CK Enzyme Mix to form an intermediate, which reduces a colorless probe to a colored product with strong absorbance at ?= 450 nm. The CK Activity Assay is high-throughput adaptable, simple and sensitive. This assay kit can detect CK activity less than 1 mU.
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Notes
Creatine Kinase (CK) also known as creatine phosphokinase (CPK) and ATP: creatine N-phosphotransferase is a common cellular enzyme (EC 2.7.3.2). It catalyzes the reversible conversion of creatine and ATP into ADP and phosphocreatine. CK is widely expressed in various tissues and cell types, with highest activity in striated muscles, heart tissue and brain. CK consists of two subunits: M (muscle) and B (brain), and has three isoenzymes: CK-MM (skeleton muscle), CK-MB (cardiac muscle), and CK-BB (brain). Increased CK level is associated with many diseases such as myocardial infarction, muscular dystrophy, pulmonary infarction and brain tumors. Accurate measurement of CK is crucial for early diagnosis, prediction and therapeutic strategy.
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components Identifier 100 tests ATP Orange 1 vial CK Assay Buffer WM 1 x 25ml CK Developer Red 1 vial CK Enzyme Mix Green 1 vial CK Positive Control Purple 1 vial CK Substrate Blue 1 x 1ml NADH Standard Yellow 1 vial -
Research areas
Images
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Functional studies - Creatine Kinase Activity Assay Kit (ab155901)Creatine Kinase Activity measured in various samples.
Initial protein concentration for tissue lysates varied from 1 mg/mL to 3 mg/mL. 40 μl of filtered tissue lysates were used. 30 μl of filtered biological fluids were used.
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Functional studies - Creatine Kinase Activity Assay KitTypical NADH standard calibration curve using colorimetric reading.
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Figure 1Creatine kinase activity tested in 5 µl human serum and 192 ng rat heart lysate. Assay performed following kit protocol.
Protocols
References
This product has been referenced in:
- Luptak I et al. Decreased ATP production and myocardial contractile reserve in metabolic heart disease. J Mol Cell Cardiol 116:106-114 (2018). Read more (PubMed: 29409987) »
- McRae N et al. Glucocorticoids Improve Myogenic Differentiation In Vitro by Suppressing the Synthesis of Versican, a Transitional Matrix Protein Overexpressed in Dystrophic Skeletal Muscles. Int J Mol Sci 18:N/A (2017). Read more (PubMed: 29211034) »
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