I used phenol red free DMEM media with 1% ITSS and 1% antibiotic/antimycotic. Dosed media was incubated with sheep cardiomyocytes for 48 hours and collected. Media was diluted 1:2 with molecular grade water and added to the kits reaction mix. Absorption readings were taken every 2 minutes for 40 minutes.
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Submitted Sep 08 2017